Abstract
The effect of pertussis toxin (PT) on transforming growth factor β1 (TGFβl)‐induced proto‐oncogene expression was investigated in AKR‐2B fibroblasts. PT substantially abolished c‐sis and c‐myc mRNA expression following TGFβl stimulation. This inhibitory effect was specific for TGFβ1‐stimulated proto‐oncogene expression and associated with the ADP‐ribosylation of a 41‐kDa substrate. Actinomycin D decay and nuclear run‐on experiments demonstrated that the inhibitory effects of PT are a result of decreased transcriptional activation and not to an increased decay of proto‐oncogene message. PT did not, however, affect TGFβl‐stimulated fibronectin and collagen mRNA accumulation nor did it have any inhibitory effect on TGFβl‐induced morphological transformation. These data indicate that TGFβl‐stimulated gene expression is coupled to multiple pathways distinguished by their sensitivity to PT.
Original language | English (US) |
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Pages (from-to) | 39-45 |
Number of pages | 7 |
Journal | Journal of Cellular Physiology |
Volume | 142 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1990 |
ASJC Scopus subject areas
- Physiology
- Clinical Biochemistry
- Cell Biology