Distinct pathways regulate transforming growth factor β1-stimulated proto-oncogene and extracellular matrix gene expression

P. H. Howe, M. R. Cunningham, Edward B Leof

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The effect of pertussis toxin (PT) on transforming growth factor β1 (TGFβ1)-induced proto-oncogene expression was investigated in AKR-2B fibroblasts. PT substantially abolished c-sis and c-myc mRNA expression following TGFβ1 stimulation. This inhibitory effect was specific for TGFβ1-stimulated proto-oncogene expression and associated with the ADP-ribosylation of a 41-kDa substrate. Actinomycin D decay and nuclear run-on experiments demonstrated that the inhibitory effects of PT are a result of decreased transcriptional activation and not of an increased decay of proto-oncogene message. PT did not, however, affect TGFβ1-stimulated fibronectin and collagen mRNA accumulation nor did it have any inhibitory effect on TGFβ1-induced morphological transformation. These data indicate that TGFβ1-stimulated gene expression is coupled to multiple pathways distinguished by their sensitivity to PT.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalJournal of Cellular Physiology
Volume142
Issue number1
StatePublished - 1990
Externally publishedYes

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Proto-Oncogenes
Transforming Growth Factors
Gene expression
Pertussis Toxin
Extracellular Matrix
Gene Expression
Messenger RNA
Dactinomycin
Fibroblasts
Fibronectins
Adenosine Diphosphate
Radioactivity
Transcriptional Activation
Collagen
Chemical activation
Substrates
Experiments

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Distinct pathways regulate transforming growth factor β1-stimulated proto-oncogene and extracellular matrix gene expression. / Howe, P. H.; Cunningham, M. R.; Leof, Edward B.

In: Journal of Cellular Physiology, Vol. 142, No. 1, 1990, p. 39-45.

Research output: Contribution to journalArticle

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