Dissociated regulation of cellular progesterone secretion and cytosolic ornithine decarboxylase activity in isolated ovarian cells in vitro

Johannes D Veldhuis, S. K. Sweinberg, P. A. Klase, J. M. Hammond

Research output: Contribution to journalArticle

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Abstract

We have utilized isolated, highly differentiated porcine granulosa cells maintained uncer chemically defined conditions in vitro to examine directly the relationship between ornithine decarboxylase (ODC) activity and progesterone secretion in ovarian cells. The administration of saturating concentrations of LH, prostaglandin E 2, L-epinephrine, or 8-bromo-cAMP each elicited highly significant increases in progesterone production, which correlated closely with corresponding stimulated levels of ODC activity. Highly purified preparations of FSH elicited no enzymatic or steroidogenic response. ODC activity and progesterone production also correlated closely after administration of various inhibitors of protein or RNA synthesis [cycloheximide (10 μg/ml), actinomycin D (1 μg/ml), or α-amanitin (1 μg/ml)]. However, in time-course experiments, progesterone secretion increased significantly before the rise in ODC activity. Furthermore, a direct irreversible catalytic inhibitor of ODC activity, DL-α-difluoromethylornithine, and three indirect inhibitors of ODC that putatively induce intracellular antizyme, produced profound suppression of enzymatic activity, without altering maximal progesterone production in response to LH. Thus, the current studies define close parallelism between hormonal regulation of ODC activity and progesterone production in ovarian cells in vitro. However, our ability to demonstrate apparent dissociation between these two processes suggests that hormonal stimulation of steroidogenesis may not be obligatorily coupled to the rapidly turning-over cytosolic protein ODC.

Original languageEnglish (US)
Pages (from-to)1657-1662
Number of pages6
JournalEndocrinology
Volume109
Issue number5
StatePublished - 1981
Externally publishedYes

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Ornithine Decarboxylase
Progesterone
Dactinomycin
Amanitins
Eflornithine
Nucleic Acid Synthesis Inhibitors
8-Bromo Cyclic Adenosine Monophosphate
Protein Synthesis Inhibitors
Granulosa Cells
Cycloheximide
Prostaglandins E
Epinephrine
In Vitro Techniques
Swine
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dissociated regulation of cellular progesterone secretion and cytosolic ornithine decarboxylase activity in isolated ovarian cells in vitro. / Veldhuis, Johannes D; Sweinberg, S. K.; Klase, P. A.; Hammond, J. M.

In: Endocrinology, Vol. 109, No. 5, 1981, p. 1657-1662.

Research output: Contribution to journalArticle

Veldhuis, Johannes D ; Sweinberg, S. K. ; Klase, P. A. ; Hammond, J. M. / Dissociated regulation of cellular progesterone secretion and cytosolic ornithine decarboxylase activity in isolated ovarian cells in vitro. In: Endocrinology. 1981 ; Vol. 109, No. 5. pp. 1657-1662.
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