Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

George Vasmatzis, Magnus Essand, Ulrich Brinkmann, Byungkook Lee, Ira Pastan

Research output: Contribution to journalArticle

131 Scopus citations

Abstract

A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.

Original languageEnglish (US)
Pages (from-to)300-304
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number1
DOIs
StatePublished - Jan 6 1998

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