Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

George Vasmatzis; Magnus Essand; Ulrich Brinkmann; Byungkook Lee; Ira Pastan

doi:10.1073/pnas.95.1.300

Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

George Vasmatzis, Magnus Essand, Ulrich Brinkmann, Byungkook Lee, Ira Pastan

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.

Original language	English (US)
Pages (from-to)	300-304
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	95
Issue number	1
DOIs	https://doi.org/10.1073/pnas.95.1.300
State	Published - Jan 6 1998

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.95.1.300

Cite this

@article{e9d0ddf13b154f1aaffafe010452e706,

title = "Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis",

abstract = "A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.",

author = "George Vasmatzis and Magnus Essand and Ulrich Brinkmann and Byungkook Lee and Ira Pastan",

year = "1998",

month = jan,

day = "6",

doi = "10.1073/pnas.95.1.300",

language = "English (US)",

volume = "95",

pages = "300--304",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "1",

}

TY - JOUR

T1 - Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

AU - Vasmatzis, George

AU - Essand, Magnus

AU - Brinkmann, Ulrich

AU - Lee, Byungkook

AU - Pastan, Ira

PY - 1998/1/6

Y1 - 1998/1/6

N2 - A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.

AB - A procedure is described to discover genes that are specifically expressed in human prostate. The procedure involves searching the expressed sequence tag (EST) database for genes that have many related EST sequences from human prostate cDNA libraries but none or few from nonprostate human libraries. The selected candidate EST clones were tested by RNA dot blots to examine tissue specificity and by Northern blots to examine the transcript size of the corresponding mRNA. The computer analysis identified 15 promising genes that were previously unidentified. When seven of these were examined in an RNA hybridization experiment, three were found to be prostate specific. The genes identified could be useful in the targeted therapy of prostate cancer. The procedure can easily be applied to discover genes specifically expressed in other organs or tumors.

UR - http://www.scopus.com/inward/record.url?scp=0031938775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031938775&partnerID=8YFLogxK

U2 - 10.1073/pnas.95.1.300

DO - 10.1073/pnas.95.1.300

M3 - Article

C2 - 9419370

AN - SCOPUS:0031938775

SN - 0027-8424

VL - 95

SP - 300

EP - 304

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 1

ER -

Discovery of three genes specifically expressed in human prostate by expressed sequence tag database analysis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this