Abstract
Brain MRI in multiple sclerosis (MS) frequently shows areas of hypointensity in the white matter on T1 weighted sequences ("T1 black holes"). These areas are thought to be consistent with irreversible axonal loss. In this study T1 black holes were characterized in Theiler's Murine Encephalitis Virus infection, an established model of demyelinating diseases in mice. The spectrum of TMEV is broad in different strains. C57BL/6J mice develop a self-limited brain disease, which resolves within 4-6 weeks. We followed six mice with serial MRI and MRS on days 0, 3,7,21 and 45. The studies were performed in a 7 Tesla magnet. Periventricular and parahippocampal T1 black holes seen as early as 3 days, with decreasing NAA/Cre ratio on MRS. The extent of pathology was most severe on days 3 and 7. T1 black holes are thought to be consistent with areas of irreversible axonal loss. This is challenged by our observations of resolution of T1 black holes by day 45. This was concomitant with the normalization of MRS findings in the areas of interest. We conclude that T1 black holes may represent a transient phenomenon in this model of MS. The recovery of these areas studied suggests an active repair mechanism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1222-1227 |
| Number of pages | 6 |
| Journal | Frontiers in Bioscience |
| Volume | 9 |
| State | Published - 2004 |
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Keywords
- MRI
- MRS
- MS models
- T1 black hole
- T1 hypointensity
- Theiler's Murine Encephalitis Virus
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Disappearing "T1 black holes" in an animal model of multiple sclerosis. / Pirko, Istvan; Johnson, Aaron J.; Gamez, Jeff; Macura, Slobodan I; Rodriguez, Moses.
In: Frontiers in Bioscience, Vol. 9, 2004, p. 1222-1227.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Disappearing "T1 black holes" in an animal model of multiple sclerosis
AU - Pirko, Istvan
AU - Johnson, Aaron J.
AU - Gamez, Jeff
AU - Macura, Slobodan I
AU - Rodriguez, Moses
PY - 2004
Y1 - 2004
N2 - Brain MRI in multiple sclerosis (MS) frequently shows areas of hypointensity in the white matter on T1 weighted sequences ("T1 black holes"). These areas are thought to be consistent with irreversible axonal loss. In this study T1 black holes were characterized in Theiler's Murine Encephalitis Virus infection, an established model of demyelinating diseases in mice. The spectrum of TMEV is broad in different strains. C57BL/6J mice develop a self-limited brain disease, which resolves within 4-6 weeks. We followed six mice with serial MRI and MRS on days 0, 3,7,21 and 45. The studies were performed in a 7 Tesla magnet. Periventricular and parahippocampal T1 black holes seen as early as 3 days, with decreasing NAA/Cre ratio on MRS. The extent of pathology was most severe on days 3 and 7. T1 black holes are thought to be consistent with areas of irreversible axonal loss. This is challenged by our observations of resolution of T1 black holes by day 45. This was concomitant with the normalization of MRS findings in the areas of interest. We conclude that T1 black holes may represent a transient phenomenon in this model of MS. The recovery of these areas studied suggests an active repair mechanism.
AB - Brain MRI in multiple sclerosis (MS) frequently shows areas of hypointensity in the white matter on T1 weighted sequences ("T1 black holes"). These areas are thought to be consistent with irreversible axonal loss. In this study T1 black holes were characterized in Theiler's Murine Encephalitis Virus infection, an established model of demyelinating diseases in mice. The spectrum of TMEV is broad in different strains. C57BL/6J mice develop a self-limited brain disease, which resolves within 4-6 weeks. We followed six mice with serial MRI and MRS on days 0, 3,7,21 and 45. The studies were performed in a 7 Tesla magnet. Periventricular and parahippocampal T1 black holes seen as early as 3 days, with decreasing NAA/Cre ratio on MRS. The extent of pathology was most severe on days 3 and 7. T1 black holes are thought to be consistent with areas of irreversible axonal loss. This is challenged by our observations of resolution of T1 black holes by day 45. This was concomitant with the normalization of MRS findings in the areas of interest. We conclude that T1 black holes may represent a transient phenomenon in this model of MS. The recovery of these areas studied suggests an active repair mechanism.
KW - MRI
KW - MRS
KW - MS models
KW - T1 black hole
KW - T1 hypointensity
KW - Theiler's Murine Encephalitis Virus
UR - http://www.scopus.com/inward/record.url?scp=2442683276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2442683276&partnerID=8YFLogxK
M3 - Article
C2 - 14977539
AN - SCOPUS:2442683276
VL - 9
SP - 1222
EP - 1227
JO - Frontiers in Bioscience - Landmark
JF - Frontiers in Bioscience - Landmark
SN - 1093-9946
ER -