Disappearing "T1 black holes" in an animal model of multiple sclerosis

Istvan Pirko, Aaron Johnson, Jeff Gamez, Slobodan I. Macura, Moses Rodriguez

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Brain MRI in multiple sclerosis (MS) frequently shows areas of hypointensity in the white matter on T1 weighted sequences ("T1 black holes"). These areas are thought to be consistent with irreversible axonal loss. In this study T1 black holes were characterized in Theiler's Murine Encephalitis Virus infection, an established model of demyelinating diseases in mice. The spectrum of TMEV is broad in different strains. C57BL/6J mice develop a self-limited brain disease, which resolves within 4-6 weeks. We followed six mice with serial MRI and MRS on days 0, 3,7,21 and 45. The studies were performed in a 7 Tesla magnet. Periventricular and parahippocampal T1 black holes seen as early as 3 days, with decreasing NAA/Cre ratio on MRS. The extent of pathology was most severe on days 3 and 7. T1 black holes are thought to be consistent with areas of irreversible axonal loss. This is challenged by our observations of resolution of T1 black holes by day 45. This was concomitant with the normalization of MRS findings in the areas of interest. We conclude that T1 black holes may represent a transient phenomenon in this model of MS. The recovery of these areas studied suggests an active repair mechanism.

Original languageEnglish (US)
Pages (from-to)1222-1227
Number of pages6
JournalFrontiers in Bioscience
Volume9
DOIs
StatePublished - 2004

Keywords

  • MRI
  • MRS
  • MS models
  • T1 black hole
  • T1 hypointensity
  • Theiler's Murine Encephalitis Virus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Disappearing "T1 black holes" in an animal model of multiple sclerosis'. Together they form a unique fingerprint.

Cite this