TY - JOUR
T1 - Directed differentiation of mouse cochlear neural progenitors in vitro
AU - Lin, Jizhen
AU - Feng, Ling
AU - Hamajima, Yuki
AU - Komori, Masahiro
AU - Burns, Terry C.
AU - Fukudome, Shinji
AU - Anderson, John
AU - Wang, Dong
AU - Verfaillie, Catherine M.
AU - Low, Walter C.
PY - 2009/3
Y1 - 2009/3
N2 - Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, whereas the differentiation of CNPs into neurons by SERB was not. This work develops a new in vitro methodology for the maintenance and self-regeneration of CNPs for future design of regenerative strategies for hearing disorders.
AB - Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, whereas the differentiation of CNPs into neurons by SERB was not. This work develops a new in vitro methodology for the maintenance and self-regeneration of CNPs for future design of regenerative strategies for hearing disorders.
KW - Growth factor
KW - Proliferation
UR - http://www.scopus.com/inward/record.url?scp=65249136542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65249136542&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.00324.2008
DO - 10.1152/ajpcell.00324.2008
M3 - Article
C2 - 19261910
AN - SCOPUS:65249136542
VL - 296
SP - C441-C452
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6143
IS - 3
ER -