Directed differentiation of mouse cochlear neural progenitors in vitro

Jizhen Lin, Ling Feng, Yuki Hamajima, Masahiro Komori, Terry C. Burns, Shinji Fukudome, John Anderson, Dong Wang, Catherine M. Verfaillie, Walter C. Low

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Multipotent cochlear neural progenitors (CNPs) in the organ of Corti hold the promise for cell replacement in degenerative hearing disorders. However, not much is known about the CNPs and the specific conditions for their differentiation. Here we isolate the CNPs from the postnatal day 1 organ of Corti in mice and demonstrate their capability to self-renew and to differentiate into hair cell-like and neuronal cell-like phenotypes under the guidance of sonic hedgehog (SHH), epidermal growth factor (EGF), retinoic acid (RA), and brain-derived neurotrophic factor (BDNF), herein termed SERB (abbreviation of SHH, EGF, RA, and BDNF) in an asymmetric or symmetric manner from clonal isolates. Differentiation of CNPs into hair cells by SERB was dependent on the ERK signaling pathway, whereas the differentiation of CNPs into neurons by SERB was not. This work develops a new in vitro methodology for the maintenance and self-regeneration of CNPs for future design of regenerative strategies for hearing disorders.

Original languageEnglish (US)
Pages (from-to)C441-C452
JournalAmerican Journal of Physiology - Cell Physiology
Volume296
Issue number3
DOIs
StatePublished - Mar 2009

Keywords

  • Growth factor
  • Proliferation

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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    Lin, J., Feng, L., Hamajima, Y., Komori, M., Burns, T. C., Fukudome, S., Anderson, J., Wang, D., Verfaillie, C. M., & Low, W. C. (2009). Directed differentiation of mouse cochlear neural progenitors in vitro. American Journal of Physiology - Cell Physiology, 296(3), C441-C452. https://doi.org/10.1152/ajpcell.00324.2008