Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture

John C Lieske, F. G. Toback, S. Deganello

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Background. The interaction of the most common crystal in human urine, calcium oxalate dihydrate (COD), with the surface of monkey renal epithelial cells (BSC-1 line) was studied to identify initiating events in kidney stone formation. Methods. To determine if COD crystals could nucleate directly onto the apical cell surface, a novel technique utilizing vapor diffusion of oxalic acid was employed. Cells were grown to confluence in the inner four wells of 24-well plates. At the start of each experiment, diethyloxalate in water was placed into eight adjacent wells, and the plates were sealed tightly with tape so that oxalic acid vapor diffused into a calcium- containing buffer overlying the cells. Results. Small crystals were visualized on the cell surface after two hours, and by six hours the unambiguous habitus of COD was confirmed. Nucleation onto cells occurred almost exclusively via the (001) face, one that is only rarely observed when COD crystals nucleate onto inanimate surfaces. Similar results were obtained when canine renal epithelial cells (MDCK line) were used as a substrate for nucleation. Initially, COD crystals were internalized almost as quickly as they formed on the apical cell surface. Conclusions. Face-specific COD crystal nucleation onto the apical surface of living renal epithelial cells followed by internalization is a heretofore unrecognized physiological event, suggesting a new mechanism to explain crystal retention within the nephron, and perhaps kidney stone formation when this process is dysregulated or overwhelmed.

Original languageEnglish (US)
Pages (from-to)796-803
Number of pages8
JournalKidney International
Volume54
Issue number3
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Calcium Oxalate
Cell Culture Techniques
Epithelial Cells
Kidney
Oxalic Acid
Kidney Calculi
Nephrons
Haplorhini
Canidae
Buffers
Urine
Calcium
Cell Line
Water

Keywords

  • Calcium oxalate
  • Cell membrane
  • Crystallization
  • Crystallography
  • Models of stones
  • Structural defects
  • X-ray of kidney calculi

ASJC Scopus subject areas

  • Nephrology

Cite this

Direct nucleation of calcium oxalate dihydrate crystals onto the surface of living renal epithelial cells in culture. / Lieske, John C; Toback, F. G.; Deganello, S.

In: Kidney International, Vol. 54, No. 3, 1998, p. 796-803.

Research output: Contribution to journalArticle

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N2 - Background. The interaction of the most common crystal in human urine, calcium oxalate dihydrate (COD), with the surface of monkey renal epithelial cells (BSC-1 line) was studied to identify initiating events in kidney stone formation. Methods. To determine if COD crystals could nucleate directly onto the apical cell surface, a novel technique utilizing vapor diffusion of oxalic acid was employed. Cells were grown to confluence in the inner four wells of 24-well plates. At the start of each experiment, diethyloxalate in water was placed into eight adjacent wells, and the plates were sealed tightly with tape so that oxalic acid vapor diffused into a calcium- containing buffer overlying the cells. Results. Small crystals were visualized on the cell surface after two hours, and by six hours the unambiguous habitus of COD was confirmed. Nucleation onto cells occurred almost exclusively via the (001) face, one that is only rarely observed when COD crystals nucleate onto inanimate surfaces. Similar results were obtained when canine renal epithelial cells (MDCK line) were used as a substrate for nucleation. Initially, COD crystals were internalized almost as quickly as they formed on the apical cell surface. Conclusions. Face-specific COD crystal nucleation onto the apical surface of living renal epithelial cells followed by internalization is a heretofore unrecognized physiological event, suggesting a new mechanism to explain crystal retention within the nephron, and perhaps kidney stone formation when this process is dysregulated or overwhelmed.

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