Direct measurement of the effect of potassium, calcium, veratridine, and and amphetamine on the rate of release of dopamine from superfused brain tissue

R. M. Wightman, Christine E. Bright, John Nathaniel Caviness

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The rate of release of endogenous DA from rat brain striatal minces has been measured using a rapid superfusion apparatus. The apparatus provides immediate, continuous readout of easily oxidized substances in the perfusate using an amperometric detector. Subsequent analysis of the perfusate (which contains pargyline) by liquid chromatography shows that the major substance detected is DA. DA release is induced by a 30 s exposure to 60 mM K+ and is Ca2+-dependent. Similar results are obtained with veratridine (10-4 M). The time resolution of the perfusion system permits discrimination of the decreased rate of release induced by veratridine (10-4 M) and amphetamine (10-5 M) as opposed to 60 mM K+. Repetitive stimulation of the striatal mince with 60 mM K+ results in a decreased amount and rate of DA release. Subsequent exposure of the striatal mince to exogenous DA results in a restoration of the K+-induced, Ca2+-dependent release, indicating uptake of DA is operant under these conditions.

Original languageEnglish (US)
Pages (from-to)1279-1286
Number of pages8
JournalLife Sciences
Volume28
Issue number11
DOIs
StatePublished - Mar 16 1981
Externally publishedYes

Fingerprint

Veratridine
Corpus Striatum
Amphetamine
Dopamine
Brain
Potassium
Tissue
Pargyline
Calcium
Liquid chromatography
Restoration
Rats
Detectors
Liquid Chromatography
Perfusion

ASJC Scopus subject areas

  • Pharmacology

Cite this

Direct measurement of the effect of potassium, calcium, veratridine, and and amphetamine on the rate of release of dopamine from superfused brain tissue. / Wightman, R. M.; Bright, Christine E.; Caviness, John Nathaniel.

In: Life Sciences, Vol. 28, No. 11, 16.03.1981, p. 1279-1286.

Research output: Contribution to journalArticle

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