Direct measurement of the effect of potassium, calcium, veratridine, and and amphetamine on the rate of release of dopamine from superfused brain tissue

R. M. Wightman, Christine E. Bright, J. N. Caviness

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The rate of release of endogenous DA from rat brain striatal minces has been measured using a rapid superfusion apparatus. The apparatus provides immediate, continuous readout of easily oxidized substances in the perfusate using an amperometric detector. Subsequent analysis of the perfusate (which contains pargyline) by liquid chromatography shows that the major substance detected is DA. DA release is induced by a 30 s exposure to 60 mM K+ and is Ca2+-dependent. Similar results are obtained with veratridine (10-4 M). The time resolution of the perfusion system permits discrimination of the decreased rate of release induced by veratridine (10-4 M) and amphetamine (10-5 M) as opposed to 60 mM K+. Repetitive stimulation of the striatal mince with 60 mM K+ results in a decreased amount and rate of DA release. Subsequent exposure of the striatal mince to exogenous DA results in a restoration of the K+-induced, Ca2+-dependent release, indicating uptake of DA is operant under these conditions.

Original languageEnglish (US)
Pages (from-to)1279-1286
Number of pages8
JournalLife Sciences
Volume28
Issue number11
DOIs
StatePublished - Mar 16 1981

ASJC Scopus subject areas

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology

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