Direct measurement of pulsatile insulin secretion from the portal vein in human subjects

Soon H. Song, Susan S. McIntyre, Hasnain Shah, Johannes D Veldhuis, Peter C. Hayes, Peter C. Butler

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Insulin is secreted in a high frequency pulsatile manner. These pulses are delivered directly into the portal vein and then undergo extraction and dilution before delivery into the systemic circulation. The reported frequency of these insulin pulses estimated in peripheral blood varies from an interpulse interval of 4-20 min. We postulated that this discrepancy is due to the attenuation of the pulse signal in the systemic circulation vs. the portal circulation. In the present study we measured pulsatile insulin release directly in the portal circulation of human subjects who had indwelling transjugular intrahepatic portasystemic stent shunts (TIPSS) to decompress portal hypertension. We quantitated pulsatile insulin secretion in both the overnight fasted state (fasting) and during a hyperglycemic clamp (8 mmol/L). Direct portal vein sampling established that pulsatile insulin secretion in humans has an interval (periodicity) of approximately 5 min. The amplitude (and mass) of the insulin concentration oscillations observed in the portal vein was approximately 5-fold greater than that observed in the arterialized vein and was similar to that observed in the dog. Increased insulin release during hyperglycemia was achieved through amplification of the insulin pulse mass. In conclusion, direct portal vein sampling in humans revealed that the interpulse interval of insulin pulses in humans is about 5 min, and this frequency is also observed when sampling from the systemic circulation using a highly specific insulin assay and 1-min sampling, but is about 4-fold greater than the frequency observed at this site using single site RIAs. We confirm that enhanced insulin release in response to hyperglycemia is achieved by amplification of these high frequency pulses.

Original languageEnglish (US)
Pages (from-to)4491-4499
Number of pages9
JournalJournal of Clinical Endocrinology and Metabolism
Volume85
Issue number12
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Portal Vein
Insulin
Sampling
Hyperglycemia
Amplification
Transjugular Intrahepatic Portasystemic Shunt
Stents
Clamping devices
Portal Hypertension
Periodicity
Dilution
Pulse
Veins
Assays
Fasting
Blood
Dogs

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Song, S. H., McIntyre, S. S., Shah, H., Veldhuis, J. D., Hayes, P. C., & Butler, P. C. (2000). Direct measurement of pulsatile insulin secretion from the portal vein in human subjects. Journal of Clinical Endocrinology and Metabolism, 85(12), 4491-4499. https://doi.org/10.1210/jc.85.12.4491

Direct measurement of pulsatile insulin secretion from the portal vein in human subjects. / Song, Soon H.; McIntyre, Susan S.; Shah, Hasnain; Veldhuis, Johannes D; Hayes, Peter C.; Butler, Peter C.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 85, No. 12, 2000, p. 4491-4499.

Research output: Contribution to journalArticle

Song, Soon H. ; McIntyre, Susan S. ; Shah, Hasnain ; Veldhuis, Johannes D ; Hayes, Peter C. ; Butler, Peter C. / Direct measurement of pulsatile insulin secretion from the portal vein in human subjects. In: Journal of Clinical Endocrinology and Metabolism. 2000 ; Vol. 85, No. 12. pp. 4491-4499.
@article{6653348bb11f43e5b64201ad807dc16b,
title = "Direct measurement of pulsatile insulin secretion from the portal vein in human subjects",
abstract = "Insulin is secreted in a high frequency pulsatile manner. These pulses are delivered directly into the portal vein and then undergo extraction and dilution before delivery into the systemic circulation. The reported frequency of these insulin pulses estimated in peripheral blood varies from an interpulse interval of 4-20 min. We postulated that this discrepancy is due to the attenuation of the pulse signal in the systemic circulation vs. the portal circulation. In the present study we measured pulsatile insulin release directly in the portal circulation of human subjects who had indwelling transjugular intrahepatic portasystemic stent shunts (TIPSS) to decompress portal hypertension. We quantitated pulsatile insulin secretion in both the overnight fasted state (fasting) and during a hyperglycemic clamp (8 mmol/L). Direct portal vein sampling established that pulsatile insulin secretion in humans has an interval (periodicity) of approximately 5 min. The amplitude (and mass) of the insulin concentration oscillations observed in the portal vein was approximately 5-fold greater than that observed in the arterialized vein and was similar to that observed in the dog. Increased insulin release during hyperglycemia was achieved through amplification of the insulin pulse mass. In conclusion, direct portal vein sampling in humans revealed that the interpulse interval of insulin pulses in humans is about 5 min, and this frequency is also observed when sampling from the systemic circulation using a highly specific insulin assay and 1-min sampling, but is about 4-fold greater than the frequency observed at this site using single site RIAs. We confirm that enhanced insulin release in response to hyperglycemia is achieved by amplification of these high frequency pulses.",
author = "Song, {Soon H.} and McIntyre, {Susan S.} and Hasnain Shah and Veldhuis, {Johannes D} and Hayes, {Peter C.} and Butler, {Peter C.}",
year = "2000",
doi = "10.1210/jc.85.12.4491",
language = "English (US)",
volume = "85",
pages = "4491--4499",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "12",

}

TY - JOUR

T1 - Direct measurement of pulsatile insulin secretion from the portal vein in human subjects

AU - Song, Soon H.

AU - McIntyre, Susan S.

AU - Shah, Hasnain

AU - Veldhuis, Johannes D

AU - Hayes, Peter C.

AU - Butler, Peter C.

PY - 2000

Y1 - 2000

N2 - Insulin is secreted in a high frequency pulsatile manner. These pulses are delivered directly into the portal vein and then undergo extraction and dilution before delivery into the systemic circulation. The reported frequency of these insulin pulses estimated in peripheral blood varies from an interpulse interval of 4-20 min. We postulated that this discrepancy is due to the attenuation of the pulse signal in the systemic circulation vs. the portal circulation. In the present study we measured pulsatile insulin release directly in the portal circulation of human subjects who had indwelling transjugular intrahepatic portasystemic stent shunts (TIPSS) to decompress portal hypertension. We quantitated pulsatile insulin secretion in both the overnight fasted state (fasting) and during a hyperglycemic clamp (8 mmol/L). Direct portal vein sampling established that pulsatile insulin secretion in humans has an interval (periodicity) of approximately 5 min. The amplitude (and mass) of the insulin concentration oscillations observed in the portal vein was approximately 5-fold greater than that observed in the arterialized vein and was similar to that observed in the dog. Increased insulin release during hyperglycemia was achieved through amplification of the insulin pulse mass. In conclusion, direct portal vein sampling in humans revealed that the interpulse interval of insulin pulses in humans is about 5 min, and this frequency is also observed when sampling from the systemic circulation using a highly specific insulin assay and 1-min sampling, but is about 4-fold greater than the frequency observed at this site using single site RIAs. We confirm that enhanced insulin release in response to hyperglycemia is achieved by amplification of these high frequency pulses.

AB - Insulin is secreted in a high frequency pulsatile manner. These pulses are delivered directly into the portal vein and then undergo extraction and dilution before delivery into the systemic circulation. The reported frequency of these insulin pulses estimated in peripheral blood varies from an interpulse interval of 4-20 min. We postulated that this discrepancy is due to the attenuation of the pulse signal in the systemic circulation vs. the portal circulation. In the present study we measured pulsatile insulin release directly in the portal circulation of human subjects who had indwelling transjugular intrahepatic portasystemic stent shunts (TIPSS) to decompress portal hypertension. We quantitated pulsatile insulin secretion in both the overnight fasted state (fasting) and during a hyperglycemic clamp (8 mmol/L). Direct portal vein sampling established that pulsatile insulin secretion in humans has an interval (periodicity) of approximately 5 min. The amplitude (and mass) of the insulin concentration oscillations observed in the portal vein was approximately 5-fold greater than that observed in the arterialized vein and was similar to that observed in the dog. Increased insulin release during hyperglycemia was achieved through amplification of the insulin pulse mass. In conclusion, direct portal vein sampling in humans revealed that the interpulse interval of insulin pulses in humans is about 5 min, and this frequency is also observed when sampling from the systemic circulation using a highly specific insulin assay and 1-min sampling, but is about 4-fold greater than the frequency observed at this site using single site RIAs. We confirm that enhanced insulin release in response to hyperglycemia is achieved by amplification of these high frequency pulses.

UR - http://www.scopus.com/inward/record.url?scp=0034489574&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034489574&partnerID=8YFLogxK

U2 - 10.1210/jc.85.12.4491

DO - 10.1210/jc.85.12.4491

M3 - Article

C2 - 11134098

AN - SCOPUS:0034489574

VL - 85

SP - 4491

EP - 4499

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 12

ER -