TY - JOUR
T1 - Direct electrochemical characterization of Vitreoscilla sp. hemoglobin entrapped in organic films
AU - Fan, Chunhai
AU - Zhong, Jun
AU - Guan, Rong
AU - Li, Genxi
N1 - Funding Information:
We thank the National Natural Science Foundation of China (Grant no. 30070214) and the Chinese Ministry of Education (Grant no. 01087) for financial support. The authors also appreciate the generous help from Dr. Qingqiu Huang (University of Science and Technology, PR China).
PY - 2003/7/30
Y1 - 2003/7/30
N2 - The redox properties of a prokaryotic, Vitreoscilla sp. hemoglobin (VHb) in fuzzy organic films are studied with electrochemistry. This VHb exhibits irreversible electrochemical response at bare pyrolytic graphite (PG) electrode surfaces. However, upon being entrapped in organic films, the heterogeneous electron transfer rate of VHb will be sufficiently high to produce a quasi-reversible electrochemical response. The observation of electrocatalysis (reduction of O2) by hemes suggests that the protein can retain its biological activity under these conditions.
AB - The redox properties of a prokaryotic, Vitreoscilla sp. hemoglobin (VHb) in fuzzy organic films are studied with electrochemistry. This VHb exhibits irreversible electrochemical response at bare pyrolytic graphite (PG) electrode surfaces. However, upon being entrapped in organic films, the heterogeneous electron transfer rate of VHb will be sufficiently high to produce a quasi-reversible electrochemical response. The observation of electrocatalysis (reduction of O2) by hemes suggests that the protein can retain its biological activity under these conditions.
KW - Vitreoscilla sp. hemoglobin (VHb)
UR - http://www.scopus.com/inward/record.url?scp=1242341363&partnerID=8YFLogxK
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U2 - 10.1016/S1570-9639(03)00162-6
DO - 10.1016/S1570-9639(03)00162-6
M3 - Article
C2 - 12878030
AN - SCOPUS:1242341363
SN - 1570-9639
VL - 1649
SP - 123
EP - 126
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 2
ER -