Direct association between the Ret receptor tyrosine kinase and the Src homology 2-containing adapter protein Grb7

Akhilesh Pandey, Xin Liu, Jack E. Dixon, Pier Paolo Di Fiore, Vishva M. Dixit

Research output: Contribution to journalArticle

75 Scopus citations

Abstract

Adapter proteins containing Src homology 2 (SH2) domains link transmembrane receptor protein-tyrosine kinases to downstream signal transducing molecules. A family of SH2 containing adapter proteins including Grb7 and Grb10 has been recently identified. We had previously shown that Grb10 associates with Ret via its SH2 domain in an activation-dependent manner (Pandey, A., Duan, H., Di Fiore, P. P., and Dixit, V. M. (1995) J. Biol. Chem. 270, 21461-21463). We now demonstrate that the related adapter molecule Grb7 also associates with Ret in vitro and in vivo, and that the binding of the SH2 domain of Grb7 to Ret is direct. This binding is dependent upon Ret autophosphorylation since Grb7 is incapable of binding a kinase- defective mutant of Ret. Thus two members of the Grb family, Grb7 and Grb10, likely relay signals emanating from Ret to other, as yet, unidentified targets within the cell.

Original languageEnglish (US)
Pages (from-to)10607-10610
Number of pages4
JournalJournal of Biological Chemistry
Volume271
Issue number18
DOIs
StatePublished - May 20 1996

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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