Direct action of estrogen on sequence of progression of human preneoplastic breast disease

M. P V Shekhar, P. Nangia-Makker, S. R. Wolman, L. Tait, G. H. Heppner, Daniel W Visscher

Research output: Contribution to journalArticle

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Abstract

We have used the MCF10AT xenograft model of human proliferative breast disease to examine the early effects of estradiol exposure on morphological progression of preneoplastic lesions and to define the step(s) in the morphological sequence at which estrogen may act. The effects of estradiol on neoplastic progression of estrogen-receptor-positive MCF10AT cells in the orthotopic site were examined in ovariectomized female nude mice that received subcutaneous administration of implants of 17β-estradiol or placebo pellets. At 10 weeks, histological analysis of the lesions derived from the estrogen-supplemented group revealed that 92% of lesions displayed histological features of atypical hyperplasia, carcinoma in situ, or invasive carcinoma, and the remaining 8% exhibited histological features of moderate hyperplasia. These highly proliferative lesions are in marked contrast to the control group in which 60% of samples displayed no evidence of hyperplasia. In contrast with control xenografts, estrogen-exposed xenografts demonstrated extensive areas of papillary growth, adenosis-like areas, prominent host inflammatory infiltration, and angiogenesis. Our results suggest that estrogen exerts a growth-promoting effect on benign or premalignant ductal epithelium by enhancing 1) the frequency of lesion formation, 2) the size of lesions, 3) the speed of transformation from normal/mild hyperplasia to those with atypia, 4) the degree of dysplasia, and 5) angiogenesis.

Original languageEnglish (US)
Pages (from-to)1129-1132
Number of pages4
JournalAmerican Journal of Pathology
Volume152
Issue number5
StatePublished - 1998
Externally publishedYes

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Breast Diseases
Hyperplasia
Estrogens
Heterografts
Estradiol
Carcinoma in Situ
Growth
Nude Mice
Estrogen Receptors
Epithelium
Placebos
Carcinoma
Control Groups

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shekhar, M. P. V., Nangia-Makker, P., Wolman, S. R., Tait, L., Heppner, G. H., & Visscher, D. W. (1998). Direct action of estrogen on sequence of progression of human preneoplastic breast disease. American Journal of Pathology, 152(5), 1129-1132.

Direct action of estrogen on sequence of progression of human preneoplastic breast disease. / Shekhar, M. P V; Nangia-Makker, P.; Wolman, S. R.; Tait, L.; Heppner, G. H.; Visscher, Daniel W.

In: American Journal of Pathology, Vol. 152, No. 5, 1998, p. 1129-1132.

Research output: Contribution to journalArticle

Shekhar, MPV, Nangia-Makker, P, Wolman, SR, Tait, L, Heppner, GH & Visscher, DW 1998, 'Direct action of estrogen on sequence of progression of human preneoplastic breast disease', American Journal of Pathology, vol. 152, no. 5, pp. 1129-1132.
Shekhar, M. P V ; Nangia-Makker, P. ; Wolman, S. R. ; Tait, L. ; Heppner, G. H. ; Visscher, Daniel W. / Direct action of estrogen on sequence of progression of human preneoplastic breast disease. In: American Journal of Pathology. 1998 ; Vol. 152, No. 5. pp. 1129-1132.
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