Dimethyl sulfoxide stimulates the catalytic activity of de novo DNA methyltransferase 3a (Dnmt3a) in vitro

Tomoki Yokochi, Keith D. Robertson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Mammalian DNA methyltransferase Dnmt3a is required for de novo methylation of CpG dinucleotides in genomic DNA. While DNA methyltransferase inhibitors have been extensively utilized both in vitro and in vivo, no stimulator of catalytic activity has been identified thus far. Here we show that the methyltransfer activity of Dnmt3a is stimulated by the addition of dimethyl sulfoxide (DMSO) to the reaction solution in vitro. Enzymatic analysis of initial reaction velocity suggests that the DMSO stimulation effect depends on the interaction between DMSO and the reaction substrates (DNA and AdoMet), but not the enzyme itself.

Original languageEnglish (US)
Pages (from-to)234-243
Number of pages10
JournalBioorganic Chemistry
Volume32
Issue number4
DOIs
StatePublished - Aug 1 2004

Keywords

  • Catalytic stimulator
  • DNA methyltransferase
  • Dimethyl sulfoxide
  • Dnmt3a
  • Enzymology
  • Kinetics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Drug Discovery
  • Organic Chemistry

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