Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative

R. Y. Kim, Kuan Hu Li Kuan Hu, Thomas J Flotte, E. S. Gragoudas, L. H Y Young

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

PURPOSE: To examine benzoporphyrin derivative angiography as a modality for studying photosensitizer biodistribution in experimental choroidal melanomas. METHODS: A liposomal preparation of benzoporphyrin derivative was used in this study. Digital benzoporphyrin derivative angiograms were performed in 10 rabbits (six for experimental choroidal melanomas, two for normal choroids, and two for irides) using a Topcon ImageNet H1024 digital imaging system, a Kodak Megaplus video camera, and a Topcon TRC-50-VT fundus camera. Only one eye from each rabbit was used. Filters specifically designed for benzoporphyrin derivative (peak absorption at 580 nm and peak emission at 695 nm) were used. Benzoporphyrin derivative (1 mg/kg) was injected into an ear vein while images of tumor, normal choroid, or iris were being obtained. Follow.up images were obtained during the first 3 hours and at 24 hours after injection. Fluorescence microscopy was performed in all 10 rabbits using 1 mg/kg of benzoporphyrin derivative. Tumor-bearing eyes were enucleated at the same time points that angiograms were performed, and the two sets of results were compared for maximum dye accumulation. RESULTS: Digital angiography demonstrated that maximal benzoporphyrin derivative fluorescence occurred in tumors 15 to 45 minutes after injection. Fluorescence photometry corroborated these results. CONCLUSION: Photosensitizer angiography is a valid modality for determining the optimum treatment time for photodynamic therapy.

Original languageEnglish (US)
Pages (from-to)810-816
Number of pages7
JournalAmerican Journal of Ophthalmology
Volume123
Issue number6
StatePublished - 1997
Externally publishedYes

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Experimental Melanomas
Angiography
Photosensitizing Agents
Choroid
Iris
Rabbits
Fluorescence
Photometry
Neoplasms
Injections
Photochemotherapy
Fluorescence Microscopy
Ear
Veins
Coloring Agents

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kim, R. Y., Li Kuan Hu, K. H., Flotte, T. J., Gragoudas, E. S., & Young, L. H. Y. (1997). Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative. American Journal of Ophthalmology, 123(6), 810-816.

Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative. / Kim, R. Y.; Li Kuan Hu, Kuan Hu; Flotte, Thomas J; Gragoudas, E. S.; Young, L. H Y.

In: American Journal of Ophthalmology, Vol. 123, No. 6, 1997, p. 810-816.

Research output: Contribution to journalArticle

Kim, RY, Li Kuan Hu, KH, Flotte, TJ, Gragoudas, ES & Young, LHY 1997, 'Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative', American Journal of Ophthalmology, vol. 123, no. 6, pp. 810-816.
Kim, R. Y. ; Li Kuan Hu, Kuan Hu ; Flotte, Thomas J ; Gragoudas, E. S. ; Young, L. H Y. / Digital angiography of experimental choroidal melanomas using benzoporphyrin derivative. In: American Journal of Ophthalmology. 1997 ; Vol. 123, No. 6. pp. 810-816.
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