Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store

Lisa Stehno-Bittel, Carmen M Terzic, David E. Clapham

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Intact, isolated nuclei and a nuclear membrane (ghost) preparation were used to study regulation of the movement of small molecules across the Xenopus laevis oocyte nuclear membrane. In contrast to models of the nuclear pore complex, which assume passive bidirectional diffusion of molecules less than 70 kilodaltons, diffusion of intermediate-sized molecules was regulated by the nuclear envelope calcium stores. After depletion of nuclear store calcium by inositol 1,4,5-trisphosphate or calcium chelators, fluorescent molecules conjugated to 10-kilodalton dextran were unable to enter the nucleus. Dye exclusion after calcium store depletion was not dependent on the nuclear matrix because it occurred in nuclear ghosts lacking nucleoplasm. Smaller molecules and ions (500-dalton Lucifer yellow and manganese) diffused freely into the core of the nuclear ghosts and intact nuclei even after calcium store depletion. Thus, depletion of the nuclear calcium store blocks diffusion of intermediate-sized molecules.

Original languageEnglish (US)
Pages (from-to)1835-1838
Number of pages4
JournalScience
Volume270
Issue number5243
StatePublished - Dec 15 1995

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Nuclear Envelope
Calcium
Nuclear Matrix
Nuclear Pore
Inositol 1,4,5-Trisphosphate
Xenopus laevis
Manganese
Dextrans
Oocytes
Coloring Agents
Ions

ASJC Scopus subject areas

  • General

Cite this

Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store. / Stehno-Bittel, Lisa; Terzic, Carmen M; Clapham, David E.

In: Science, Vol. 270, No. 5243, 15.12.1995, p. 1835-1838.

Research output: Contribution to journalArticle

Stehno-Bittel, L, Terzic, CM & Clapham, DE 1995, 'Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store', Science, vol. 270, no. 5243, pp. 1835-1838.
Stehno-Bittel, Lisa ; Terzic, Carmen M ; Clapham, David E. / Diffusion across the nuclear envelope inhibited by depletion of the nuclear Ca2+ store. In: Science. 1995 ; Vol. 270, No. 5243. pp. 1835-1838.
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