Differentiation of llc-pki cells cultured on native (contractible) collagen gels

C. R. Sussman, J. L. Renfro

Research output: Contribution to journalArticle

Abstract

The porcine-derived renal epithelial cell line, LLC-PKi, forms a functional epithelium with apical tight junctions and reabsorptive Na+/glucose colransport. In an attempt to further differentiate this epithelium we cultured these cells on contraclible collagen gels. Transepithelial electrical potential difference (PD), resistance (R) and total (It), phloridzin-sensitive (Iphz, 0.18 mM phloridzin), and glucosedependent (Iglc) currents were measured in Ussing chambers. LLCPK1 cells contracted collagen gels from 32 mm to 22± 0.2 mm in 18 days. Gels were contracted 1 mm/day for the first 10 days then -(1.5 mm/day. Four days after plating confluent monolayers exhibited R = 38.4 ±3.0 fixem2, PD = -0.8 ±0.1 mV, It = 20.2 ±0.8 /iA/cm2, and IphJ. - 14-1 ±1. μA. Over the next 15 days It and IphJ. increased approximately 5 fold. R decreased -50% between 10 and 11 days after plating, and PD increased -80% between 8 and 9 days and another -50% between 14 and 15 days. 17-19 day old cultures had R = 18.0 ± 0.6 Qxcm2, PD = -2.0 ±0.1 niv, 11 = 101.7 ±2.0//A/cm2, Iphz = 69.8 ± 2.4 μA/cm2, and Iglc = 88.4 ±1.5 μA/cm2. LLC-PKi cells cultured on contractible collagen had transport capabilities far better than those previously observed on non-contractible collagen-coated filters. Electrical characteristics were comparable to those of rat and rabbit proximal tubule in vivo. Supported by NSF.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

Phlorhizin
phloridzin
cultured cells
collagen
Cultured Cells
Collagen
Gels
gels
Plating
epithelium
Epithelium
proximal tubules
tight junctions
Tight Junctions
kidney cells
Rats
Monolayers
epithelial cells
Swine
Epithelial Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Differentiation of llc-pki cells cultured on native (contractible) collagen gels. / Sussman, C. R.; Renfro, J. L.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Sussman, C. R. ; Renfro, J. L. / Differentiation of llc-pki cells cultured on native (contractible) collagen gels. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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