Differential spatial approximation between secretin and its receptor residues in active and inactive conformations demonstrated by photoaffinity labeling

Maoqing Dong, Keiko Hosohata, Delia I. Pinon, Natesa Muthukumaraswamy, Laurence J Miller

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Understanding of the conformational changes in G protein-coupled receptors associated with activation and inactivation is of great interest. We previously used photoaffinity labeling to elucidate spatial approximations between photolabile residues situated throughout the pharmacophore of secretin agonist probes and this receptor. The aim of the current work was to develop analogous photolabile secretin antagonist probes and to explore their spatial approximations. The most potent secretin antagonist reported is a pseudopeptide ([ψ4, 5]secretin) in which the peptide bond between residues 4 and 5 was replaced by a ψ(CH2-NH) peptide bond isostere. We have developed a series of [ψ4, 5]secretin analogs incorporating photolabile benzoyl phenylalanine residues in positions 6, 22, and 26. Each bound to the secretin receptor saturably and specifically, with affinity similar to their parental peptide. At concentrations with no measurable agonist activity, each probe covalently labeled the secretin receptor. Peptide mapping using proteolytic cleavage, immunoprecipitation, and radiochemical sequencing identified that each of these three probes labeled the amino terminus of the secretin receptor. Whereas the position 22 probe labeled the same residue as its analogous agonist probe and the position 6 probe labeled a residue within two residues of that labeled by its analogous agonist probe, the position 26 probe labeled a site 16 residues away from that labeled by its analogous agonist probe. Thus, whereas structurally related agonist and antagonist probes dock in the same general region of this receptor, conformational differences in active and inactive states result in substantial differences in spatial approximation at the carboxyl-terminal end of secretin analogs.

Original languageEnglish (US)
Pages (from-to)1688-1698
Number of pages11
JournalMolecular Endocrinology
Volume20
Issue number7
DOIs
StatePublished - 2006

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Secretin
Peptides
Peptide Mapping
G-Protein-Coupled Receptors
Phenylalanine
Immunoprecipitation
secretin receptor

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Differential spatial approximation between secretin and its receptor residues in active and inactive conformations demonstrated by photoaffinity labeling. / Dong, Maoqing; Hosohata, Keiko; Pinon, Delia I.; Muthukumaraswamy, Natesa; Miller, Laurence J.

In: Molecular Endocrinology, Vol. 20, No. 7, 2006, p. 1688-1698.

Research output: Contribution to journalArticle

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