Differential sensitivity of fibroblasts to epidermal growth factor is related to cyclic AMP concentration

N. E. Olashaw, Edward B Leof, E. J. O'Keefe, W. J. Pledger

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The differential sensitivity of various cell lines to the mitogenic effects of epidermal growth factor (EGF) was investigated. Two lines of evidence suggest that cellular capacity to respond proliferatively to EGF is related to intracellular cyclic AMP concentration. First, the ability of three density-arrested cell lines to synthesize DNA in response to EGF was directly proportional to the basal cyclic AMP level of the cells at quiescence. Second, treatment of cultures with various agents known to promote intracellular cyclic AMP accumulation increased the sensitivity of all three cell lines to EGF. The mechanism whereby cyclic AMP modulates EGF responsiveness is not known; cholera toxin did not affect the cellular capacity to bind or internalize and process EGF. Although platelet-derived growth factor (PDGF) had no effect on cyclic AMP levels, transient treatment of quiescent cultures with this polypeptide also enhanced EGF sensitivity. In agreement with previous data and in contrast to cholera toxin, PDGF induced the down-regulation of EGF receptors in the three cell lines. These data suggest that the capacity of various cell types to respond to EGF is subject to both intracellular regulation by cyclic AMP and extracellular modulation by factors such as PDGF which can affect EGF receptor activity.

Original languageEnglish (US)
Pages (from-to)291-297
Number of pages7
JournalJournal of Cellular Physiology
Volume118
Issue number3
StatePublished - 1984
Externally publishedYes

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Fibroblasts
Epidermal Growth Factor
Cyclic AMP
Platelet-Derived Growth Factor
Cells
Cell Line
Cholera Toxin
Epidermal Growth Factor Receptor
Down-Regulation
Modulation
Peptides
DNA

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Differential sensitivity of fibroblasts to epidermal growth factor is related to cyclic AMP concentration. / Olashaw, N. E.; Leof, Edward B; O'Keefe, E. J.; Pledger, W. J.

In: Journal of Cellular Physiology, Vol. 118, No. 3, 1984, p. 291-297.

Research output: Contribution to journalArticle

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