Abstract
Although all three Vav family members are expressed in T lymphocytes, the role that Vav3 plays in T cell activation is poorly defined. Here we show that, like Vavl, Vav3 undergoes rapid tyrosine phosphorylation after T cell receptor (TCR) cross-linkage and interacts with the adaptor molecules SLP76 and 3BP2 in a SH2-dependent manner. However, depletion of Vav1 but not Vav3 protein by RNA interference affects TCR-mediated IL-2 promoter activity. In contrast, Vav3 function is specifically required for coupling TCR stimulation to serum response element-mediated gene transcription. These data indicate that, although both Vav proteins are biochemically coupled to the TCR, they regulate distinct molecular pathways leading to defined gene transcriptional events.
Original language | English (US) |
---|---|
Pages (from-to) | 429-434 |
Number of pages | 6 |
Journal | Journal of Experimental Medicine |
Volume | 199 |
Issue number | 3 |
DOIs | |
State | Published - Feb 2 2004 |
Keywords
- GEF
- SRE
- Signal transduction
- T cell
- Vav
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology