TY - JOUR
T1 - Differential proteomic expression in indolent versus transforming oral lichen planus
AU - Xie, Fangyi
AU - Gleue, Casey A.
AU - Deschaine, Maria
AU - Dasari, Surendra
AU - Sartori-Valinotti, Julio C.
AU - Charlesworth, M. Cristine
AU - Meves, Alexander
AU - Lehman, Julia
N1 - Funding Information:
This research was supported by the Appignani Lichen Planus Benefactor Gift at Mayo Clinic. Dr Xie is supported by the British Association of Dermatologists Geoffrey Dowling Fellowship for her visiting research fellowship. The Proteomics Core is a shared resource of the Mayo Clinic Cancer Center (NCI P30 CA15083).
Publisher Copyright:
© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
PY - 2023
Y1 - 2023
N2 - Oral lichen planus (OLP) confers an approximately 1% risk of transformation to oral squamous cell carcinoma (OSCC). Early identification of high-risk OLP would be very helpful for optimal patient management. We aimed to discover specific tissue-based protein biomarkers in patients with OLP who developed OSCC compared to those who did not. We used laser capture microdissection– and nanoLC–tandem mass spectrometry to assess protein expression in fixed lesional mucosal specimens in patients with indolent OLP (no OSCC after at least 5-year follow-up, n = 6), transforming OLP (non-dysplastic epithelium with lichenoid inflammation marginal to OSCC, n = 6) or normal oral mucosa (NOM, n = 5). Transforming OLP protein profile was enriched for actin cytoskeleton, mitochondrial dysfunction and oxidative phosphorylation pathways. CA1, TNNT3, SYNM and MB were overexpressed, and FBLN1 was underexpressed in transforming OLP compared with indolent OLP. Integrin signalling and antigen presentation pathways were enriched in both indolent and transforming OLP compared with NOM. This proteomic study provides potential biomarkers, such as CA1 overexpression, for higher-risk OLP. While further validation studies are needed, we propose that epithelial–mesenchymal transition may be involved in OLP carcinogenesis.
AB - Oral lichen planus (OLP) confers an approximately 1% risk of transformation to oral squamous cell carcinoma (OSCC). Early identification of high-risk OLP would be very helpful for optimal patient management. We aimed to discover specific tissue-based protein biomarkers in patients with OLP who developed OSCC compared to those who did not. We used laser capture microdissection– and nanoLC–tandem mass spectrometry to assess protein expression in fixed lesional mucosal specimens in patients with indolent OLP (no OSCC after at least 5-year follow-up, n = 6), transforming OLP (non-dysplastic epithelium with lichenoid inflammation marginal to OSCC, n = 6) or normal oral mucosa (NOM, n = 5). Transforming OLP protein profile was enriched for actin cytoskeleton, mitochondrial dysfunction and oxidative phosphorylation pathways. CA1, TNNT3, SYNM and MB were overexpressed, and FBLN1 was underexpressed in transforming OLP compared with indolent OLP. Integrin signalling and antigen presentation pathways were enriched in both indolent and transforming OLP compared with NOM. This proteomic study provides potential biomarkers, such as CA1 overexpression, for higher-risk OLP. While further validation studies are needed, we propose that epithelial–mesenchymal transition may be involved in OLP carcinogenesis.
KW - lichen planus
KW - malignant transformation
KW - oral lichen planus
KW - oral squamous cell carcinoma
KW - proteomics
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U2 - 10.1111/exd.14738
DO - 10.1111/exd.14738
M3 - Article
C2 - 36587284
AN - SCOPUS:85146170769
SN - 0906-6705
JO - Experimental Dermatology
JF - Experimental Dermatology
ER -