Differential gene expression between young and senescent, quiescent WI-38 cells

David L. Doggett, Mitch O. Rotenberg, Robert J. Pignolo, Paul D. Phillips, Vincent J. Cristofalo

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

To investigate age-related changes in gene expression in WI-38 cells, we isolated RNA from young and senescent, quiescent cultures and made subtracted cDNA libraries. Density-arrested cells were incubated in serum-free MCDB-104 for 3 days. RNA was then isolated and subtracted cDNA libraries were made in the phagemid vector pCDM8. Both by picking clones at random from these subtracted libraries and by differential hybridization screening with subtracted cDNA probes from young and senescent cells, we have identified a total of 11 genes for which RNA is expressed differentially in these quiescent young and senescent WI-38 cultures. Two genes, EPC-1 and EPC-A2, with elevated RNA levels in young cells, have sequences which have not previously been identified. Two of the genes with elevated RNA expression in the senescent cells are the mitochodnria-coded genes for NADH dehydrogenase subunit 4 and for cytochrome b. We also identified seven other genes with elevated RNA levels in senescent cells. Three of these, LPC-1, LPC-14 and LPC-24, have been partially sequenced and have not previously been identified. These studies show that density-arrested, serum-deprived, quiescent young and senescent cells express a number of genes differentially. These differences are not growth-dependent, but are age-dependent. Our studies also show that the methods employed here, which include careful regulation of the cell cultures and subtraction of the libraries, result in libraries from which differentially expressed genes can be identified, either by random selection or by differential hybridization screening with subtracted probes.

Original languageEnglish (US)
Pages (from-to)239-255
Number of pages17
JournalMechanisms of Ageing and Development
Volume65
Issue number2-3
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

Fingerprint Dive into the research topics of 'Differential gene expression between young and senescent, quiescent WI-38 cells'. Together they form a unique fingerprint.

Cite this