Differential expression of interferon-induced genes and other tissue-based biomarkers in acute graft-versus-host disease vs. lupus erythematosus in skin

Julia Lehman, Surendra Dasari, S. S. Damodaran, R. A. el-Azhary, L. E. Gibson, S. K. Hashmi, William Hogan, Saad Kenderian, Mrinal M Patnaik, Mark R Litzow, H. M. Lazarus, Alexander Meves

Research output: Contribution to journalArticle

Abstract

Background: In both acute graft-versus-host disease (GVHD) and lupus erythematosus (LE), the patient's own tissues are subjected to immunological assault via complex mechanisms influenced by interferon (IFN) and other cytokines. Although not typically confused clinically, these entities have overlapping histopathological findings in the skin. Aim: To assess whether GVHD can be differentiated from LE using molecular methods on skin specimens. Methods: We developed a quantitative reverse transcription PCR assay based on previously identified tissue-based biomarkers of cutaneous GVHD, and compared gene expression in GVHD with that in LE. Results: Both entities showed robust expression of IFN-induced genes and of genes encoding proteins involved in antigen presentation, cell signalling and tissue repair. Levels of gene expression differed significantly in GVHD compared with LE, particularly those of IFN-induced genes such as MX1, OAS3, TAP1 and STAT3 (P < 0.01). Three logistic regression models could differentiate the two entities with a high degree of certainty (receiver operating characteristic area under the curve of 1.0). Conclusion: The study demonstrates the feasibility of distinguishing between microscopically similar inflammatory dermatoses using tissue-based molecular techniques.

Original languageEnglish (US)
JournalClinical and Experimental Dermatology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Graft vs Host Disease
Interferons
Biomarkers
Skin
Genes
Logistic Models
Gene Expression
Antigen Presentation
Feasibility Studies
Skin Diseases
ROC Curve
Reverse Transcription
Area Under Curve
Cytokines
Polymerase Chain Reaction
Proteins

ASJC Scopus subject areas

  • Dermatology

Cite this

@article{de72aa11849b44f7a998ed045c33dce9,
title = "Differential expression of interferon-induced genes and other tissue-based biomarkers in acute graft-versus-host disease vs. lupus erythematosus in skin",
abstract = "Background: In both acute graft-versus-host disease (GVHD) and lupus erythematosus (LE), the patient's own tissues are subjected to immunological assault via complex mechanisms influenced by interferon (IFN) and other cytokines. Although not typically confused clinically, these entities have overlapping histopathological findings in the skin. Aim: To assess whether GVHD can be differentiated from LE using molecular methods on skin specimens. Methods: We developed a quantitative reverse transcription PCR assay based on previously identified tissue-based biomarkers of cutaneous GVHD, and compared gene expression in GVHD with that in LE. Results: Both entities showed robust expression of IFN-induced genes and of genes encoding proteins involved in antigen presentation, cell signalling and tissue repair. Levels of gene expression differed significantly in GVHD compared with LE, particularly those of IFN-induced genes such as MX1, OAS3, TAP1 and STAT3 (P < 0.01). Three logistic regression models could differentiate the two entities with a high degree of certainty (receiver operating characteristic area under the curve of 1.0). Conclusion: The study demonstrates the feasibility of distinguishing between microscopically similar inflammatory dermatoses using tissue-based molecular techniques.",
author = "Julia Lehman and Surendra Dasari and Damodaran, {S. S.} and el-Azhary, {R. A.} and Gibson, {L. E.} and Hashmi, {S. K.} and William Hogan and Saad Kenderian and Patnaik, {Mrinal M} and Litzow, {Mark R} and Lazarus, {H. M.} and Alexander Meves",
year = "2018",
month = "1",
day = "1",
doi = "10.1111/ced.13759",
language = "English (US)",
journal = "Clinical and Experimental Dermatology",
issn = "0307-6938",
publisher = "Wiley-Blackwell",

}

TY - JOUR

T1 - Differential expression of interferon-induced genes and other tissue-based biomarkers in acute graft-versus-host disease vs. lupus erythematosus in skin

AU - Lehman, Julia

AU - Dasari, Surendra

AU - Damodaran, S. S.

AU - el-Azhary, R. A.

AU - Gibson, L. E.

AU - Hashmi, S. K.

AU - Hogan, William

AU - Kenderian, Saad

AU - Patnaik, Mrinal M

AU - Litzow, Mark R

AU - Lazarus, H. M.

AU - Meves, Alexander

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Background: In both acute graft-versus-host disease (GVHD) and lupus erythematosus (LE), the patient's own tissues are subjected to immunological assault via complex mechanisms influenced by interferon (IFN) and other cytokines. Although not typically confused clinically, these entities have overlapping histopathological findings in the skin. Aim: To assess whether GVHD can be differentiated from LE using molecular methods on skin specimens. Methods: We developed a quantitative reverse transcription PCR assay based on previously identified tissue-based biomarkers of cutaneous GVHD, and compared gene expression in GVHD with that in LE. Results: Both entities showed robust expression of IFN-induced genes and of genes encoding proteins involved in antigen presentation, cell signalling and tissue repair. Levels of gene expression differed significantly in GVHD compared with LE, particularly those of IFN-induced genes such as MX1, OAS3, TAP1 and STAT3 (P < 0.01). Three logistic regression models could differentiate the two entities with a high degree of certainty (receiver operating characteristic area under the curve of 1.0). Conclusion: The study demonstrates the feasibility of distinguishing between microscopically similar inflammatory dermatoses using tissue-based molecular techniques.

AB - Background: In both acute graft-versus-host disease (GVHD) and lupus erythematosus (LE), the patient's own tissues are subjected to immunological assault via complex mechanisms influenced by interferon (IFN) and other cytokines. Although not typically confused clinically, these entities have overlapping histopathological findings in the skin. Aim: To assess whether GVHD can be differentiated from LE using molecular methods on skin specimens. Methods: We developed a quantitative reverse transcription PCR assay based on previously identified tissue-based biomarkers of cutaneous GVHD, and compared gene expression in GVHD with that in LE. Results: Both entities showed robust expression of IFN-induced genes and of genes encoding proteins involved in antigen presentation, cell signalling and tissue repair. Levels of gene expression differed significantly in GVHD compared with LE, particularly those of IFN-induced genes such as MX1, OAS3, TAP1 and STAT3 (P < 0.01). Three logistic regression models could differentiate the two entities with a high degree of certainty (receiver operating characteristic area under the curve of 1.0). Conclusion: The study demonstrates the feasibility of distinguishing between microscopically similar inflammatory dermatoses using tissue-based molecular techniques.

UR - http://www.scopus.com/inward/record.url?scp=85054304963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054304963&partnerID=8YFLogxK

U2 - 10.1111/ced.13759

DO - 10.1111/ced.13759

M3 - Article

JO - Clinical and Experimental Dermatology

JF - Clinical and Experimental Dermatology

SN - 0307-6938

ER -