Differential expression of human globin genes introduced in K562 cells.

D. Kioussis, F. Wilson, Khashayarsha Khazaie, F. Grosveld

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We have constructed a series of hybrid globin genes between the 5' half of the human epsilon-, gamma- and beta-globin genes, and the 3' half of the rabbit beta-globin gene. These hybrid genes were introduced into the cell line K562. Analysis of the hybrid mRNA populations show that only the hybrid epsilon- and gamma-globin genes are expressed in these cells in concordance with the endogenous K562 globin gene expression. It is therefore likely that these cells contain transcriptional factors which allow the specific expression of introduced epsilon- and gamma-, but not beta-globin genes, irrespective of their chromosomal localization.

Original languageEnglish (US)
Pages (from-to)927-931
Number of pages5
JournalEMBO Journal
Volume4
Issue number4
StatePublished - Apr 1985
Externally publishedYes

Fingerprint

Globins
K562 Cells
Genes
beta-Globins
epsilon-Globins
gamma-Globins
Gene expression
Cells
Rabbits
Gene Expression
Cell Line
Messenger RNA
Population

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Kioussis, D., Wilson, F., Khazaie, K., & Grosveld, F. (1985). Differential expression of human globin genes introduced in K562 cells. EMBO Journal, 4(4), 927-931.

Differential expression of human globin genes introduced in K562 cells. / Kioussis, D.; Wilson, F.; Khazaie, Khashayarsha; Grosveld, F.

In: EMBO Journal, Vol. 4, No. 4, 04.1985, p. 927-931.

Research output: Contribution to journalArticle

Kioussis, D, Wilson, F, Khazaie, K & Grosveld, F 1985, 'Differential expression of human globin genes introduced in K562 cells.', EMBO Journal, vol. 4, no. 4, pp. 927-931.
Kioussis, D. ; Wilson, F. ; Khazaie, Khashayarsha ; Grosveld, F. / Differential expression of human globin genes introduced in K562 cells. In: EMBO Journal. 1985 ; Vol. 4, No. 4. pp. 927-931.
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