Differential expression and activity of p34(cdc2) in cultured aortic adventitial fibroblasts derived from spontaneously hypertensive and Wistar-Kyoto rats

S. L. Venance, M. H. Watson, Dennis A Wigle, A. S. Mak, S. C. Pang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: The present investigation was undertaken to determine whether p34(cdc2), a cell-cycle regulatory kinase, is involved in the manifestation of the altered proliferation evident in fibroblasts isolated from spontaneously hypertensive rats (SHR). Design: Experiments were performed on quiescent aortic adventitial fibroblasts stimulated to re-enter the cell cycle in order to examine the timing of cell cycle-related events. Methods: The cell-cycle phase was determined by flow cytometry and was related to the cellular content and kinase activity of p34(cdc2). Results: SHR fibroblasts displayed a heightened basal level of p34(cdc2) at quiescence relative to Wistar-Kyoto (WKY) rat cells. Both SHR and WKY fibroblasts showed a cell cycle-dependent increase in p34(cdc2) content, beginning in S phase. However, the SHR adventitial fibroblasts exited G0-G1 several hours earlier than the WKY fibroblasts as indicated by the time of initiation of DNA synthesis and increase in activity of p34(cdc2). Conclusions: SHR aortic adventitial fibroblasts appear to have a heightened proliferative capacity relative to WKY fibroblasts, which is evident in a quicker exit from G0 and faster transition to DNA synthesis, followed by the earlier activation of p34(cdc2).

Original languageEnglish (US)
Pages (from-to)483-489
Number of pages7
JournalJournal of Hypertension
Volume11
Issue number5
DOIs
StatePublished - 1993
Externally publishedYes

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Adventitia
Inbred WKY Rats
Fibroblasts
Inbred SHR Rats
Cell Cycle
Phosphotransferases
DNA
S Phase
Flow Cytometry

Keywords

  • Adventitial fibroblasts
  • Cell cycle
  • p34(cdc2)
  • Proliferation
  • Spontaneously hypertensive rat

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology

Cite this

Differential expression and activity of p34(cdc2) in cultured aortic adventitial fibroblasts derived from spontaneously hypertensive and Wistar-Kyoto rats. / Venance, S. L.; Watson, M. H.; Wigle, Dennis A; Mak, A. S.; Pang, S. C.

In: Journal of Hypertension, Vol. 11, No. 5, 1993, p. 483-489.

Research output: Contribution to journalArticle

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T1 - Differential expression and activity of p34(cdc2) in cultured aortic adventitial fibroblasts derived from spontaneously hypertensive and Wistar-Kyoto rats

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AU - Watson, M. H.

AU - Wigle, Dennis A

AU - Mak, A. S.

AU - Pang, S. C.

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AB - Objective: The present investigation was undertaken to determine whether p34(cdc2), a cell-cycle regulatory kinase, is involved in the manifestation of the altered proliferation evident in fibroblasts isolated from spontaneously hypertensive rats (SHR). Design: Experiments were performed on quiescent aortic adventitial fibroblasts stimulated to re-enter the cell cycle in order to examine the timing of cell cycle-related events. Methods: The cell-cycle phase was determined by flow cytometry and was related to the cellular content and kinase activity of p34(cdc2). Results: SHR fibroblasts displayed a heightened basal level of p34(cdc2) at quiescence relative to Wistar-Kyoto (WKY) rat cells. Both SHR and WKY fibroblasts showed a cell cycle-dependent increase in p34(cdc2) content, beginning in S phase. However, the SHR adventitial fibroblasts exited G0-G1 several hours earlier than the WKY fibroblasts as indicated by the time of initiation of DNA synthesis and increase in activity of p34(cdc2). Conclusions: SHR aortic adventitial fibroblasts appear to have a heightened proliferative capacity relative to WKY fibroblasts, which is evident in a quicker exit from G0 and faster transition to DNA synthesis, followed by the earlier activation of p34(cdc2).

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