Differential effects of prenatal and postnatal nutritional environment on β-cell mass development and turnover in male and female rats

Aleksey V Matveyenko, Inderroop Singh, Bo Chul Shin, Senta Georgia, Sherin U. Devaskar

Research output: Contribution to journalArticle

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Abstract

Fetal nutrient and growth restriction is associated with development of type 2 diabetes. Although the exact mechanisms responsible for this association remain debated, intrauterine and/or postnatal maldevelopment of β-cell mass has been proposed as a potential mechanism. To address this hypothesis, β-cell mass development and turnover was assessed in rats exposed to either intrauterine and/or postnatal caloric/growth restriction. In total, four groups of male and female Sprague Dawley rats (n = 69) were developed and studied: 1) control rats, i.e. control mothers rearing control pups; 2) intrauterine calorically and growth-restricted rats, i.e. 50% prenatal calorically restricted pups cross-fostered to control mothers; 3) postnatal calorically and growth-restricted rats, i.e. 50% calorically restricted mothers rearing pups born to control mothers; and 4) prenatal and postnatal calorically and growth restricted rats, i.e.50%calorically restricted mothers rearing intrauterine 50% calorically restricted pups. Intrauterine growth restriction resulted in approximately 45% reduction of postnatal β-cell fractional area and mass characterized by reduced rate of β-cell replication and decreased evidence of neogenesis. In contrast, β-cell fractional area and weight-adjusted β-cell mass in postnatal growth restriction was approximately 30% higher than in control rats. Rats exposed to both intrauterine and postnatal caloric and growth restriction demonstrated approximately 80% decrease in β-cell mass, reduction in β-cell replication, and decreased evidence of neogenesis compared with control. Neither intrauterine nor postnatal caloric restriction significantly affected the rate of β-cell apoptosis. These data support the hypothesis that intrauterine maldevelopment of β-cell mass may predict the increased risk of type 2 diabetes in adult life.

Original languageEnglish (US)
Pages (from-to)5647-5656
Number of pages10
JournalEndocrinology
Volume151
Issue number12
DOIs
StatePublished - Dec 2010
Externally publishedYes

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Caloric Restriction
Growth
Type 2 Diabetes Mellitus
Fetal Development
Sprague Dawley Rats
Apoptosis
Weights and Measures
Food

ASJC Scopus subject areas

  • Endocrinology

Cite this

Differential effects of prenatal and postnatal nutritional environment on β-cell mass development and turnover in male and female rats. / Matveyenko, Aleksey V; Singh, Inderroop; Shin, Bo Chul; Georgia, Senta; Devaskar, Sherin U.

In: Endocrinology, Vol. 151, No. 12, 12.2010, p. 5647-5656.

Research output: Contribution to journalArticle

Matveyenko, Aleksey V ; Singh, Inderroop ; Shin, Bo Chul ; Georgia, Senta ; Devaskar, Sherin U. / Differential effects of prenatal and postnatal nutritional environment on β-cell mass development and turnover in male and female rats. In: Endocrinology. 2010 ; Vol. 151, No. 12. pp. 5647-5656.
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abstract = "Fetal nutrient and growth restriction is associated with development of type 2 diabetes. Although the exact mechanisms responsible for this association remain debated, intrauterine and/or postnatal maldevelopment of β-cell mass has been proposed as a potential mechanism. To address this hypothesis, β-cell mass development and turnover was assessed in rats exposed to either intrauterine and/or postnatal caloric/growth restriction. In total, four groups of male and female Sprague Dawley rats (n = 69) were developed and studied: 1) control rats, i.e. control mothers rearing control pups; 2) intrauterine calorically and growth-restricted rats, i.e. 50{\%} prenatal calorically restricted pups cross-fostered to control mothers; 3) postnatal calorically and growth-restricted rats, i.e. 50{\%} calorically restricted mothers rearing pups born to control mothers; and 4) prenatal and postnatal calorically and growth restricted rats, i.e.50{\%}calorically restricted mothers rearing intrauterine 50{\%} calorically restricted pups. Intrauterine growth restriction resulted in approximately 45{\%} reduction of postnatal β-cell fractional area and mass characterized by reduced rate of β-cell replication and decreased evidence of neogenesis. In contrast, β-cell fractional area and weight-adjusted β-cell mass in postnatal growth restriction was approximately 30{\%} higher than in control rats. Rats exposed to both intrauterine and postnatal caloric and growth restriction demonstrated approximately 80{\%} decrease in β-cell mass, reduction in β-cell replication, and decreased evidence of neogenesis compared with control. Neither intrauterine nor postnatal caloric restriction significantly affected the rate of β-cell apoptosis. These data support the hypothesis that intrauterine maldevelopment of β-cell mass may predict the increased risk of type 2 diabetes in adult life.",
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