Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity

Laura Vanagas, María Candelaria de La Fuente, Marianela Dalghi, Mariela Ferreira-Gomes, Rolando C. Rossi, Emanuel E. Strehler, Irene C. Mangialavori, Juan P F C Rossi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641-1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the relationship between the polymerization state of actin and its effects on purified PMCA activity. Our results show that PMCA associates with the actin cytoskeleton and this interaction causes a modulation of the catalytic activity involving the phosphorylated intermediate of the pump. The state of actin polymerization determines whether it acts as an activator or an inhibitor of the pump: G-actin and/or short oligomers activate the pump, while F-actin inhibits it. The effects of actin on PMCA are the consequence of direct interaction as demonstrated by immunoblotting and cosedimentation experiments. Taken together, these findings suggest that interactions with actin play a dynamic role in the regulation of PMCA-mediated Ca2+ extrusion through the membrane. Our results provide further evidence of the activation-inhibition phenomenon as a property of many cytoskeleton-associated membrane proteins where the cytoskeleton is no longer restricted to a mechanical function but is dynamically involved in modulating the activity of integral proteins with which it interacts.

Original languageEnglish (US)
Pages (from-to)187-198
Number of pages12
JournalCell Biochemistry and Biophysics
Volume66
Issue number1
DOIs
StatePublished - 2013

Fingerprint

Plasma Membrane Calcium-Transporting ATPases
Cell membranes
Actins
Cell Membrane
Pumps
Calcium
Cytoskeleton
Actin Cytoskeleton
Polymerization
Membrane Proteins
Immunoblotting
Oligomers
Extrusion
Catalyst activity
Membranes
Chemical activation
Modulation

Keywords

  • Actin
  • Cytoskeleton
  • PMCA
  • Regulation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Vanagas, L., de La Fuente, M. C., Dalghi, M., Ferreira-Gomes, M., Rossi, R. C., Strehler, E. E., ... Rossi, J. P. F. C. (2013). Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity. Cell Biochemistry and Biophysics, 66(1), 187-198. https://doi.org/10.1007/s12013-012-9467-6

Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity. / Vanagas, Laura; de La Fuente, María Candelaria; Dalghi, Marianela; Ferreira-Gomes, Mariela; Rossi, Rolando C.; Strehler, Emanuel E.; Mangialavori, Irene C.; Rossi, Juan P F C.

In: Cell Biochemistry and Biophysics, Vol. 66, No. 1, 2013, p. 187-198.

Research output: Contribution to journalArticle

Vanagas, L, de La Fuente, MC, Dalghi, M, Ferreira-Gomes, M, Rossi, RC, Strehler, EE, Mangialavori, IC & Rossi, JPFC 2013, 'Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity', Cell Biochemistry and Biophysics, vol. 66, no. 1, pp. 187-198. https://doi.org/10.1007/s12013-012-9467-6
Vanagas L, de La Fuente MC, Dalghi M, Ferreira-Gomes M, Rossi RC, Strehler EE et al. Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity. Cell Biochemistry and Biophysics. 2013;66(1):187-198. https://doi.org/10.1007/s12013-012-9467-6
Vanagas, Laura ; de La Fuente, María Candelaria ; Dalghi, Marianela ; Ferreira-Gomes, Mariela ; Rossi, Rolando C. ; Strehler, Emanuel E. ; Mangialavori, Irene C. ; Rossi, Juan P F C. / Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity. In: Cell Biochemistry and Biophysics. 2013 ; Vol. 66, No. 1. pp. 187-198.
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