Differential effect of pH upon cyclic-ADP-ribose and nicotinate - adenine dinucleotide phosphate-induced Ca2+ release systems

Eduardo N. Chini, Mingyu Liang, Thomas P. Dousa

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We investigated the pH dependence and the effects of thimerosal and dithiothreitol (DTT) upon the Ca2+ release induced by cADP-ribose (cADPR) and nicotinate-adenine dinucleotide phosphate (NAADP) in sea urchin egg homogenates. Both Ca2+ release triggered by cADPR and the binding of [3H]cADPR to sea urchin egg homogenates were decreased by alkalization of the assay media from pH 7.2 to 8.9. In contrast, NAADP-triggered Ca2+ release was not influenced by changes in pH. The Ca2+ release induced by cADPR was potentiated by thimerosal and inhibited by DTT, but neither thimerosal nor DTT had any effect upon the Ca2+ release induced by NAADP. We conclude that cADPR-sensitive Ca2+-release mechanisms are dependent on pH of the assay media and are sensitive to thiol group modification. On the other hand, these functional properties are not shared by NAADP-regulated Ca2+ channels.

Original languageEnglish (US)
Pages (from-to)499-504
Number of pages6
JournalBiochemical Journal
Volume335
Issue number3
DOIs
StatePublished - Nov 1 1998

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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