Different thermostabilities of sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPases from rabbit and trout muscles

F. G.S. de Toledo, M. C. Albuquerque, B. H. Goulart, E. N. Chini

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Trout and rabbit (Ca2+ + Mg2+)-ATPases from sarcoplasmic reticulum were compared for differences in thermal inactivation and susceptibility to trypsin digestion. The trout ATPase is more heat-sensitive than the rabbit ATPase and is stabilized by Ca2+, Na+, K+ and nucleotides. Solubilization of both ATPases shows that the two ATPases have different protein-intrinsic inactivation kinetics. When digested by trypsin, the two ATPases display different cleavage patterns. The present results indicate that the trout and rabbit ATPases have dissimilarities in protein structure that may explain the differences in thermal inactivation kinetics.

Original languageEnglish (US)
Pages (from-to)93-98
Number of pages6
JournalComparative Biochemistry and Physiology. Part C: Comparative
Issue number1
StatePublished - May 1995


  • (Ca + Mg)-ATPase
  • Conformational states
  • Muscle
  • Rabbit
  • Rainbow trout
  • Sarcoplasmic reticulum
  • Temperature adaptation

ASJC Scopus subject areas

  • Immunology
  • Pharmacology


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