Differences in the susceptibility of MHC and non-MHC mixed lymphocyte reactions to suppression by murine amnionic fluid and its components

C. J. Krco, E. Johnson, C. S. David, T. B. Tomasi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The ability of mouse amniotic fluid (MAF), alpha-foetoprotein (AFP) and MAF depleted of AFP (MAF - AFP) to suppress primary one way MLR's was investigated. It was found that MAF, AFP, and MAF - AFP were all suppressive of MLR's specific for MHC, K, D or I + S determinants. Suppression was observed when either lymph node or spleen cells were used as the responder cells. Nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. In contrast, MLR's specific for non-MHC/M-locus determinants demonstrated either diminished suppression or augmentation of the response, compared with the MHC stimulated MLR's. Our results show a differential effect of whole MAF and its fractions on the proliferative responses induced by various allogeneic stimuli and suggest that suppression is not due to a non-specific effect on proliferation regardless of the stimulus or cell type involved.

Original languageEnglish (US)
Pages (from-to)439-446
Number of pages8
JournalJournal of Immunogenetics
Volume6
Issue number6
StatePublished - 1979

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Mixed Lymphocyte Culture Test
Amniotic Fluid
alpha-Fetoproteins
Minor Lymphocyte Stimulatory Loci
Wool
Nylons
Immunosuppressive Agents
Spleen
Lymph Nodes

ASJC Scopus subject areas

  • Genetics
  • Immunology

Cite this

Differences in the susceptibility of MHC and non-MHC mixed lymphocyte reactions to suppression by murine amnionic fluid and its components. / Krco, C. J.; Johnson, E.; David, C. S.; Tomasi, T. B.

In: Journal of Immunogenetics, Vol. 6, No. 6, 1979, p. 439-446.

Research output: Contribution to journalArticle

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