Differences in neuronal and glial cell phenotypic expression in neuron-glia cocultures: Influence of glia-conditioned media and living glial cell substrata

Kendall H Lee, Susan Kentroti, Antonia Vernadakis

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Neuronglia cocultures were prepared using, as a source for glial cells, either C6 glia (2B clone) of early (2B23) or late (2B111) passages or advanced passages of glial cells derived from primary cultures prepared from aged mouse cerebral hemispheres (MACH). Sixday-old chick embryo cerebral hemispheres (E6CH) were the source of neuron-enriched cultures. Glutamine synthetase (GS) activity was used as a marker for astrocytes and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) activity was used as a marker for oligodendrocytes. GS activity was markedly enhanced in cocultures of E6CH neurons and 2B23 glioblastic cells, whereas GS activity was reduced in cocultures of E6CH neurons and 2B111 astrocytic glia. In contrast, CNP activity was enhanced in cocultures of C6 glial cells with E6CH neurons. Glial cells from aged mouse brain did not respond to coculturing with E6CH neurons. It appears from these findings that neuronal input enhances the differentiation of glioblastic cells to either astrocytic or oligodendrocytic expression, whereas it decreases the activity of committed astrocytes. In contrast, glial cells from aged mouse brain do not respond to neuronal input. Choline acetyltransferase (ChAT) activity, a marker for cholinergic neurons, was enhanced only when E6CH cultures were grown in conditioned medium (CM) from 2B23 glioblastic cells. In contrast, ChAT activity was markedly diminished when E6CH neurons were cocultured with MACH glial cells but not when grown in CM from MACH glial cells. Thus, humoral factors from immature glial cells appear to enhance cholinergic neuronal phenotypic expression whereas cell-cell membrane contacts with aged glial cells diminish cholinergic phenotypic expression. The findings present supportive evidence that neuron-glia interrelationships are age dependent.

Original languageEnglish (US)
Pages (from-to)861-870
Number of pages10
JournalBrain Research Bulletin
Volume28
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Conditioned Culture Medium
Coculture Techniques
Neuroglia
Neurons
nucleotidase
Glutamate-Ammonia Ligase
Choline O-Acetyltransferase
Cyclic Nucleotides
Cerebrum
Astrocytes
Cholinergic Agents
Cholinergic Neurons
Oligodendroglia
Brain
Chick Embryo
Cell Differentiation
Clone Cells
Cell Membrane

Keywords

  • C-6 glia Aged brain mouse glia Neuron-glia interactions Humoral factors Cell-cell contacts Cholinergic neurons Astrocytes Oligodendrocytes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differences in neuronal and glial cell phenotypic expression in neuron-glia cocultures : Influence of glia-conditioned media and living glial cell substrata. / Lee, Kendall H; Kentroti, Susan; Vernadakis, Antonia.

In: Brain Research Bulletin, Vol. 28, No. 6, 1992, p. 861-870.

Research output: Contribution to journalArticle

@article{57e99a956a794eeba3cee03cc1ef02db,
title = "Differences in neuronal and glial cell phenotypic expression in neuron-glia cocultures: Influence of glia-conditioned media and living glial cell substrata",
abstract = "Neuronglia cocultures were prepared using, as a source for glial cells, either C6 glia (2B clone) of early (2B23) or late (2B111) passages or advanced passages of glial cells derived from primary cultures prepared from aged mouse cerebral hemispheres (MACH). Sixday-old chick embryo cerebral hemispheres (E6CH) were the source of neuron-enriched cultures. Glutamine synthetase (GS) activity was used as a marker for astrocytes and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) activity was used as a marker for oligodendrocytes. GS activity was markedly enhanced in cocultures of E6CH neurons and 2B23 glioblastic cells, whereas GS activity was reduced in cocultures of E6CH neurons and 2B111 astrocytic glia. In contrast, CNP activity was enhanced in cocultures of C6 glial cells with E6CH neurons. Glial cells from aged mouse brain did not respond to coculturing with E6CH neurons. It appears from these findings that neuronal input enhances the differentiation of glioblastic cells to either astrocytic or oligodendrocytic expression, whereas it decreases the activity of committed astrocytes. In contrast, glial cells from aged mouse brain do not respond to neuronal input. Choline acetyltransferase (ChAT) activity, a marker for cholinergic neurons, was enhanced only when E6CH cultures were grown in conditioned medium (CM) from 2B23 glioblastic cells. In contrast, ChAT activity was markedly diminished when E6CH neurons were cocultured with MACH glial cells but not when grown in CM from MACH glial cells. Thus, humoral factors from immature glial cells appear to enhance cholinergic neuronal phenotypic expression whereas cell-cell membrane contacts with aged glial cells diminish cholinergic phenotypic expression. The findings present supportive evidence that neuron-glia interrelationships are age dependent.",
keywords = "C-6 glia Aged brain mouse glia Neuron-glia interactions Humoral factors Cell-cell contacts Cholinergic neurons Astrocytes Oligodendrocytes",
author = "Lee, {Kendall H} and Susan Kentroti and Antonia Vernadakis",
year = "1992",
doi = "10.1016/0361-9230(92)90206-D",
language = "English (US)",
volume = "28",
pages = "861--870",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Differences in neuronal and glial cell phenotypic expression in neuron-glia cocultures

T2 - Influence of glia-conditioned media and living glial cell substrata

AU - Lee, Kendall H

AU - Kentroti, Susan

AU - Vernadakis, Antonia

PY - 1992

Y1 - 1992

N2 - Neuronglia cocultures were prepared using, as a source for glial cells, either C6 glia (2B clone) of early (2B23) or late (2B111) passages or advanced passages of glial cells derived from primary cultures prepared from aged mouse cerebral hemispheres (MACH). Sixday-old chick embryo cerebral hemispheres (E6CH) were the source of neuron-enriched cultures. Glutamine synthetase (GS) activity was used as a marker for astrocytes and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) activity was used as a marker for oligodendrocytes. GS activity was markedly enhanced in cocultures of E6CH neurons and 2B23 glioblastic cells, whereas GS activity was reduced in cocultures of E6CH neurons and 2B111 astrocytic glia. In contrast, CNP activity was enhanced in cocultures of C6 glial cells with E6CH neurons. Glial cells from aged mouse brain did not respond to coculturing with E6CH neurons. It appears from these findings that neuronal input enhances the differentiation of glioblastic cells to either astrocytic or oligodendrocytic expression, whereas it decreases the activity of committed astrocytes. In contrast, glial cells from aged mouse brain do not respond to neuronal input. Choline acetyltransferase (ChAT) activity, a marker for cholinergic neurons, was enhanced only when E6CH cultures were grown in conditioned medium (CM) from 2B23 glioblastic cells. In contrast, ChAT activity was markedly diminished when E6CH neurons were cocultured with MACH glial cells but not when grown in CM from MACH glial cells. Thus, humoral factors from immature glial cells appear to enhance cholinergic neuronal phenotypic expression whereas cell-cell membrane contacts with aged glial cells diminish cholinergic phenotypic expression. The findings present supportive evidence that neuron-glia interrelationships are age dependent.

AB - Neuronglia cocultures were prepared using, as a source for glial cells, either C6 glia (2B clone) of early (2B23) or late (2B111) passages or advanced passages of glial cells derived from primary cultures prepared from aged mouse cerebral hemispheres (MACH). Sixday-old chick embryo cerebral hemispheres (E6CH) were the source of neuron-enriched cultures. Glutamine synthetase (GS) activity was used as a marker for astrocytes and 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) activity was used as a marker for oligodendrocytes. GS activity was markedly enhanced in cocultures of E6CH neurons and 2B23 glioblastic cells, whereas GS activity was reduced in cocultures of E6CH neurons and 2B111 astrocytic glia. In contrast, CNP activity was enhanced in cocultures of C6 glial cells with E6CH neurons. Glial cells from aged mouse brain did not respond to coculturing with E6CH neurons. It appears from these findings that neuronal input enhances the differentiation of glioblastic cells to either astrocytic or oligodendrocytic expression, whereas it decreases the activity of committed astrocytes. In contrast, glial cells from aged mouse brain do not respond to neuronal input. Choline acetyltransferase (ChAT) activity, a marker for cholinergic neurons, was enhanced only when E6CH cultures were grown in conditioned medium (CM) from 2B23 glioblastic cells. In contrast, ChAT activity was markedly diminished when E6CH neurons were cocultured with MACH glial cells but not when grown in CM from MACH glial cells. Thus, humoral factors from immature glial cells appear to enhance cholinergic neuronal phenotypic expression whereas cell-cell membrane contacts with aged glial cells diminish cholinergic phenotypic expression. The findings present supportive evidence that neuron-glia interrelationships are age dependent.

KW - C-6 glia Aged brain mouse glia Neuron-glia interactions Humoral factors Cell-cell contacts Cholinergic neurons Astrocytes Oligodendrocytes

UR - http://www.scopus.com/inward/record.url?scp=0026729236&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026729236&partnerID=8YFLogxK

U2 - 10.1016/0361-9230(92)90206-D

DO - 10.1016/0361-9230(92)90206-D

M3 - Article

C2 - 1353404

AN - SCOPUS:0026729236

VL - 28

SP - 861

EP - 870

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 6

ER -