Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection

Shuang Wang, Li chun Lü, Hong Li, Pei zhou Jiang, Kai tai Yao

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To study the gene expression profile of human polymeric immunoglobulin receptor gene (hpIgR)-transfected mouse nasopharyngeal epithelial cells transformed with n, n'-dinitrosoperazine (TMNE) before and after EBV infection using cDNA array and investigate the role of Epstein-Barr virus (EBV) infection in the tumorigenesis of nasopharyngeal carcinoma (NPC). METHODS: The total RNAs of hpIgR-transfected TMNE cells before and after EBV infection were extracted, reversely transcribed, and labeled with alpha -(32)P-dATP. The cDNA probes were hybridized to the Atlas mouse cancer array 1.2, and the signals analyzed by AtlasImage software. RESULTS: Twenty-five genes differentially expressed in cells before and after EBV infection, including 23 up-regulated genes and 2 down-regulated genes. CONCLUSIONS: The gene expression profile of hpIgR-transfected TMNE cells may change after EBV infection, suggesting that these genes are probably involved in the tumorigenesis and progression of NPC.

Original languageEnglish (US)
Pages (from-to)1001-1005
Number of pages5
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume24
Issue number9
StatePublished - Sep 2004

ASJC Scopus subject areas

  • Medicine(all)

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