Abstract
OBJECTIVE: To study the gene expression profile of human polymeric immunoglobulin receptor gene (hpIgR)-transfected mouse nasopharyngeal epithelial cells transformed with n, n'-dinitrosoperazine (TMNE) before and after EBV infection using cDNA array and investigate the role of Epstein-Barr virus (EBV) infection in the tumorigenesis of nasopharyngeal carcinoma (NPC). METHODS: The total RNAs of hpIgR-transfected TMNE cells before and after EBV infection were extracted, reversely transcribed, and labeled with alpha -(32)P-dATP. The cDNA probes were hybridized to the Atlas mouse cancer array 1.2, and the signals analyzed by AtlasImage software. RESULTS: Twenty-five genes differentially expressed in cells before and after EBV infection, including 23 up-regulated genes and 2 down-regulated genes. CONCLUSIONS: The gene expression profile of hpIgR-transfected TMNE cells may change after EBV infection, suggesting that these genes are probably involved in the tumorigenesis and progression of NPC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1001-1005 |
| Number of pages | 5 |
| Journal | Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA |
| Volume | 24 |
| Issue number | 9 |
| State | Published - Sep 2004 |
ASJC Scopus subject areas
- Medicine(all)