Dietary reversal of experimental hypercholesterolemia improves endothelial dysfunction of epicardial arteries but not of small coronary vessels in pigs

Katherine J E Sattler, Offer Galili, Martin G Rodriguez-Porcel, James D. Krier, Lilach O Lerman, Amir Lerman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Endothelial dysfunction is characterized by impaired vasodilation, increase of oxidative stress and inflammation. The current study was designed to test the hypothesis that reversal of hypercholesterolemic diet alone does not normalize all the parameters of endothelial dysfunction. After 10 weeks on a high-cholesterol diet, female juvenile pigs were randomized to normal diet (n = 5, "Reversals") or continued on the same diet (n = 6, "HC") for another 6 weeks. A control group of 11 pigs received a normal diet ("C"). Coronary epicardial and arteriolar endothelial function was tested in vitro. NFκB and p47phox expression was analyzed in epicardial arteries and myocardium, respectively. P47phox localization in coronary arteries was demonstrated with immunohistochemistry. Lipid levels normalized in Reversal pigs. Epicardial arteries of Reversals showed a normalized relaxation and NFκB expression compared to HC (p < 0.05). Small vessel relaxation remained attenuated, and expression of p47phox in myocardial tissue was elevated in Reversals compared to C (p < 0.05). Dietary lowering of serum cholesterol and LDL improves vascular function of epicardial arteries but neither of small vessels nor vascular oxidative stress within this time frame. Hence, dietary normalization of serum lipid levels alone may not be synonymous to normalization of the components of endothelial dysfunction.

Original languageEnglish (US)
Pages (from-to)301-308
Number of pages8
JournalAtherosclerosis
Volume188
Issue number2
DOIs
StatePublished - Oct 2006

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Hypercholesterolemia
Coronary Vessels
Swine
Arteries
Diet
Blood Vessels
Oxidative Stress
Lipids
Serum
Vasodilation
LDL Cholesterol
Myocardium
Immunohistochemistry
Cholesterol
Inflammation
Control Groups

Keywords

  • Cholesterol
  • Nuclear factor κB
  • Small vessels
  • Vasorelaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Dietary reversal of experimental hypercholesterolemia improves endothelial dysfunction of epicardial arteries but not of small coronary vessels in pigs. / Sattler, Katherine J E; Galili, Offer; Rodriguez-Porcel, Martin G; Krier, James D.; Lerman, Lilach O; Lerman, Amir.

In: Atherosclerosis, Vol. 188, No. 2, 10.2006, p. 301-308.

Research output: Contribution to journalArticle

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