Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis

Faraz Bishehsari, Phillip A. Engen, Nailliw Z. Preite, Yunus E. Tuncil, Ankur Naqib, Maliha Shaikh, Marco Rossi, Sherry Wilber, Stefan J. Green, Bruce R. Hamaker, Khashayarsha Khazaie, Robin M. Voigt, Christopher B. Forsyth, Ali Keshavarzian

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Epidemiological studies propose a protective role for dietary fiber in colon cancer (CRC). One possible mechanism of fiber is its fermentation property in the gut and ability to change microbiota composition and function. Here, we investigate the role of a dietary fiber mixture in polyposis and elucidate potential mechanisms using TS4Cre×cAPCl°×468 mice. Stool microbiota profiling was performed, while functional prediction was done using PICRUSt. Stool short-chain fatty acid (SCFA) metabolites were measured. Histone acetylation and expression of SCFA butyrate receptor were assessed. We found that SCFA-producing bacteria were lower in the polyposis mice, suggesting a decline in the fermentation product of dietary fibers with polyposis. Next, a high fiber diet was given to polyposis mice, which significantly increased SCFA-producing bacteria as well as SCFA levels. This was associated with an increase in SCFA butyrate receptor and a significant decrease in polyposis. In conclusion, we found polyposis to be associated with dysbiotic microbiota characterized by a decline in SCFA-producing bacteria, which was targetable by high fiber treatment, leading to an increase in SCFA levels and amelioration of polyposis. The prebiotic activity of fiber, promoting beneficial bacteria, could be the key mechanism for the protective effects of fiber on colon carcinogenesis. SCFA-promoting fermentable fibers are a promising dietary intervention to prevent CRC.

Original languageEnglish (US)
Article number102
JournalGenes
Volume9
Issue number2
DOIs
StatePublished - Feb 16 2018

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Volatile Fatty Acids
Dietary Fiber
Colon
Carcinogenesis
Microbiota
Bacteria
Butyrates
Fermentation
Gastrointestinal Microbiome
Prebiotics
Acetylation
Histones
Colonic Neoplasms
Epidemiologic Studies
Diet

Keywords

  • Butyrate
  • CRC
  • Dietary fiber
  • Microbiota
  • SCFA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Bishehsari, F., Engen, P. A., Preite, N. Z., Tuncil, Y. E., Naqib, A., Shaikh, M., ... Keshavarzian, A. (2018). Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis. Genes, 9(2), [102]. https://doi.org/10.3390/genes9020102

Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis. / Bishehsari, Faraz; Engen, Phillip A.; Preite, Nailliw Z.; Tuncil, Yunus E.; Naqib, Ankur; Shaikh, Maliha; Rossi, Marco; Wilber, Sherry; Green, Stefan J.; Hamaker, Bruce R.; Khazaie, Khashayarsha; Voigt, Robin M.; Forsyth, Christopher B.; Keshavarzian, Ali.

In: Genes, Vol. 9, No. 2, 102, 16.02.2018.

Research output: Contribution to journalArticle

Bishehsari, F, Engen, PA, Preite, NZ, Tuncil, YE, Naqib, A, Shaikh, M, Rossi, M, Wilber, S, Green, SJ, Hamaker, BR, Khazaie, K, Voigt, RM, Forsyth, CB & Keshavarzian, A 2018, 'Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis', Genes, vol. 9, no. 2, 102. https://doi.org/10.3390/genes9020102
Bishehsari, Faraz ; Engen, Phillip A. ; Preite, Nailliw Z. ; Tuncil, Yunus E. ; Naqib, Ankur ; Shaikh, Maliha ; Rossi, Marco ; Wilber, Sherry ; Green, Stefan J. ; Hamaker, Bruce R. ; Khazaie, Khashayarsha ; Voigt, Robin M. ; Forsyth, Christopher B. ; Keshavarzian, Ali. / Dietary fiber treatment corrects the composition of gut microbiota, promotes SCFA production, and suppresses colon carcinogenesis. In: Genes. 2018 ; Vol. 9, No. 2.
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