TY - JOUR
T1 - Dietary antioxidants preserve endothelium dependent vasorelaxation in overfed rats
AU - Sato, Junichi
AU - O'Brien, Timothy
AU - Katusic, Zvonimir S.
AU - Fu, Aizhong
AU - Nygren, Jonas
AU - Singh, Ravinder
AU - Nair, K. Sreekumaran
N1 - Funding Information:
The work was supported by Mayo Clinic intramural research grants (Timothy O'Brien) and in part by the National Institutes of Health grants HL-44116, HL-53542 (Zvonimir S. Katusic), HL-58080 (Timothy O'Brien). This work was also supported by The Swedish Society of Medicine, The Medical Research Council, Henning and Johan Throne–Holsts Foundation, and by Wenner–Gren Center Foundation (Jonas Nygren).
PY - 2002
Y1 - 2002
N2 - Objective: A high fat diet contributes to obesity and acutely impairs endothelium dependent vasorelaxation. While a high cholesterol diet chronically impairs endothelium dependent vasorelaxation in rabbits, this model is associated with severe hypercholesterolemia. The effect of chronic high fat feeding on endothelial function in the setting of more normal lipid levels has not been studied. Our aim was to study vascular function in rats overfed for 6 months and to determine the role of oxidative stress in the alteration of vascular function associated with this diet. Methods: Forty-five male Sprague-Dawley rats were placed on the following diets for 6 months, control diet, high fat diet or high fat diet supplemented with vitamins A, E and selenium. Six months later blood samples were collected and vascular function was assessed in the aorta. Results: The rats fed a high fat diet were heavier than controls (608.4 ± 41.8 vs. 700.3 ± 50.1 vs. 699.5 ± 52.6 g, P < 0.05 for control vs. high fat and high fat plus antioxidant groups) but lipid levels were similar in each group (cholesterol, 145.9 ± 53.4 vs. 140.5 ± 44.0 vs. 152.7 ± 36.1 mg/dl and triglycerides, 173.2 ± 106.7 vs. 197.4 ± 131.3 vs. 166.1 ± 65.3 mg/dl, P, NS). Relaxations to acetylcholine and calcium were significantly impaired in the high fat diet group compared with controls (EC50, 6.90 ± 0.22, 7.12 ± 0.32; AUC, 96.9 ± 51.6, 155.5 ± 73.7) but were not different between the antioxidant supplemented group and controls (EC50, 7.06 ± 0.37; AUC, 151.9 ± 67.4). Relaxations to DEA NONOate were similar in each group. Conclusions: Dietary antioxidants preserved endothelium dependent vasorelaxation in rats fed a high fat diet for 6 months.
AB - Objective: A high fat diet contributes to obesity and acutely impairs endothelium dependent vasorelaxation. While a high cholesterol diet chronically impairs endothelium dependent vasorelaxation in rabbits, this model is associated with severe hypercholesterolemia. The effect of chronic high fat feeding on endothelial function in the setting of more normal lipid levels has not been studied. Our aim was to study vascular function in rats overfed for 6 months and to determine the role of oxidative stress in the alteration of vascular function associated with this diet. Methods: Forty-five male Sprague-Dawley rats were placed on the following diets for 6 months, control diet, high fat diet or high fat diet supplemented with vitamins A, E and selenium. Six months later blood samples were collected and vascular function was assessed in the aorta. Results: The rats fed a high fat diet were heavier than controls (608.4 ± 41.8 vs. 700.3 ± 50.1 vs. 699.5 ± 52.6 g, P < 0.05 for control vs. high fat and high fat plus antioxidant groups) but lipid levels were similar in each group (cholesterol, 145.9 ± 53.4 vs. 140.5 ± 44.0 vs. 152.7 ± 36.1 mg/dl and triglycerides, 173.2 ± 106.7 vs. 197.4 ± 131.3 vs. 166.1 ± 65.3 mg/dl, P, NS). Relaxations to acetylcholine and calcium were significantly impaired in the high fat diet group compared with controls (EC50, 6.90 ± 0.22, 7.12 ± 0.32; AUC, 96.9 ± 51.6, 155.5 ± 73.7) but were not different between the antioxidant supplemented group and controls (EC50, 7.06 ± 0.37; AUC, 151.9 ± 67.4). Relaxations to DEA NONOate were similar in each group. Conclusions: Dietary antioxidants preserved endothelium dependent vasorelaxation in rats fed a high fat diet for 6 months.
KW - Dilation
KW - Endothelial function
KW - Free radicals
KW - Nitric oxide
KW - Nutrition
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U2 - 10.1016/S0021-9150(01)00649-9
DO - 10.1016/S0021-9150(01)00649-9
M3 - Article
C2 - 11888515
AN - SCOPUS:0036121233
SN - 0021-9150
VL - 161
SP - 327
EP - 333
JO - Atherosclerosis
JF - Atherosclerosis
IS - 2
ER -