Diaphragm motor unit recruitment in rats

Carlos B. Mantilla; Yasin B. Seven; Wen Zhi Zhan; Gary C. Sieck

doi:10.1016/j.resp.2010.07.001

Diaphragm motor unit recruitment in rats

Carlos B. Mantilla, Yasin B. Seven, Wen Zhi Zhan, Gary C. Sieck

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97 Scopus citations

Abstract

We hypothesized that considerable force reserve exists for the diaphragm muscle (DIAm) to generate transdiaphragmatic pressures (Pdi) necessary to sustain ventilation. In rats, we measured Pdi and DIAm EMG activity during different ventilatory (eupnea and hypoxia (10% O₂)-hypercapnia (5% CO₂)) and non-ventilatory (airway occlusion and sneezing induced by intranasal capsaicin) behaviors. Compared to maximum Pdi (Pdi_max generated by bilateral phrenic nerve stimulation), the Pdi generated during eupnea (21±2%) and hypoxia-hypercapnia (28±4%) were significantly less (p<0.0001) than that generated during airway occlusion (63±4%) and sneezing (94±5%). The Pdi generated during spontaneous sighs was 62±5% of Pdi_max. Relative DIAm EMG activity (root mean square [RMS] amplitude) paralleled the changes in Pdi during different ventilatory and non-ventilatory behaviors (r²=0.78; p<0.0001). These results support our hypothesis of a considerable force reserve for the DIAm to accomplish ventilatory behaviors. A model for DIAm motor unit recruitment predicted that ventilatory behaviors would require activation of only fatigue resistant units.

Original language	English (US)
Pages (from-to)	101-106
Number of pages	6
Journal	Respiratory Physiology and Neurobiology
Volume	173
Issue number	1
DOIs	https://doi.org/10.1016/j.resp.2010.07.001
State	Published - Aug 2010

Keywords

Motor units
Phrenic nerve
Recruitment order
Respiration

ASJC Scopus subject areas

General Neuroscience
Physiology
Pulmonary and Respiratory Medicine

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10.1016/j.resp.2010.07.001

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title = "Diaphragm motor unit recruitment in rats",

abstract = "We hypothesized that considerable force reserve exists for the diaphragm muscle (DIAm) to generate transdiaphragmatic pressures (Pdi) necessary to sustain ventilation. In rats, we measured Pdi and DIAm EMG activity during different ventilatory (eupnea and hypoxia (10% O2)-hypercapnia (5% CO2)) and non-ventilatory (airway occlusion and sneezing induced by intranasal capsaicin) behaviors. Compared to maximum Pdi (Pdimax generated by bilateral phrenic nerve stimulation), the Pdi generated during eupnea (21±2%) and hypoxia-hypercapnia (28±4%) were significantly less (p<0.0001) than that generated during airway occlusion (63±4%) and sneezing (94±5%). The Pdi generated during spontaneous sighs was 62±5% of Pdimax. Relative DIAm EMG activity (root mean square [RMS] amplitude) paralleled the changes in Pdi during different ventilatory and non-ventilatory behaviors (r2=0.78; p<0.0001). These results support our hypothesis of a considerable force reserve for the DIAm to accomplish ventilatory behaviors. A model for DIAm motor unit recruitment predicted that ventilatory behaviors would require activation of only fatigue resistant units.",

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Diaphragm motor unit recruitment in rats

Abstract

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