Diaphragm capillarity and oxidative capacity during postnatal development

Gary C Sieck, T. S. Cheung, C. E. Blanco

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the cat diaphragm, fiber capillarity, cross-sectional area, and succinate dehydrogenase (SDH) activity were measured across the first 6 wk of postnatal development. Fibers were classified as type I, IIa, IIb, or IIc on the basis of staining for myofibrillar adenosinetriphosphatase (ATPase). Capillaries were identified in sections stained for ATPase at pH 4.2. Fiber cross-sectional areas and SDH activities were quantified using an image-processing system. During postnatal development, the proportions of type I fibers increased while type II fibers decreased. At birth, all type II fibers were IIc. From the 1st to the 2nd postnatal wk, the proportion of type IIc fibers decreased while the numbers of IIa and IIb increased. Thereafter the proportion of type IIb fibers continued to increase while the number of IIa steadily declined. At birth, capillarity, cross-sectional areas, and SDH activities of type I and II fibers were low compared with other postnatal age groups. Fiber cross-sectional areas increased progressively with age. The number of capillaries surrounding type I and II fibers increased markedly by the 2nd wk and then continued to increase at a slower rate. The number of capillaries per fiber area reached a peak by the 2nd wk and then declined as fiber cross-sectional area increased. Postnatal changes in capillarity depended on fiber type, being greatest in IIb. SDH activities of type I and II fibers were initially low during the first 2 postnatal wk and then peaked by the 3rd wk. After the 6th wk, fiber SDH activities decreased to adult values. Among the type II fibers, IIb showed the greatest change in SDH activity during early postnatal development.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalJournal of Applied Physiology
Volume70
Issue number1
StatePublished - 1991
Externally publishedYes

Fingerprint

Capillary Action
Succinate Dehydrogenase
Diaphragm
Adenosine Triphosphatases
Parturition
Cats
Age Groups
Staining and Labeling

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Sieck, G. C., Cheung, T. S., & Blanco, C. E. (1991). Diaphragm capillarity and oxidative capacity during postnatal development. Journal of Applied Physiology, 70(1), 103-111.

Diaphragm capillarity and oxidative capacity during postnatal development. / Sieck, Gary C; Cheung, T. S.; Blanco, C. E.

In: Journal of Applied Physiology, Vol. 70, No. 1, 1991, p. 103-111.

Research output: Contribution to journalArticle

Sieck, GC, Cheung, TS & Blanco, CE 1991, 'Diaphragm capillarity and oxidative capacity during postnatal development', Journal of Applied Physiology, vol. 70, no. 1, pp. 103-111.
Sieck, Gary C ; Cheung, T. S. ; Blanco, C. E. / Diaphragm capillarity and oxidative capacity during postnatal development. In: Journal of Applied Physiology. 1991 ; Vol. 70, No. 1. pp. 103-111.
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