Diagnostic utility of fluorescence in situ hybridization in mantle-cell lymphoma

E. D. Remstein, P. J. Kurtin, I. Buño, R. J. Bailey, J. Proffitt, W. A. Wyatt, C. A. Hanson, Gordon W. Dewald

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

Mantle-cell lymphoma (MCL) has a poorer prognosis than other small B-cell lymphomas, thus a definitive diagnosis is essential. The t(11;14)(q13;q32) associated with MCL juxtaposes portions of CCND1 (11q13) and IGH (14q32), resulting in over-expression of cyclin D1. In this study, a highly sensitive two-colour fluorescence in situ hybridization (FISH) method was developed to detect t(11;14)(q13;q32) in nuclei isolated from paraffin-embedded tissue. Twenty-three MCLs, 13 normal controls and nine small B-cell lymphomas other than MCL were studied by FISH. Each MCL had been previously investigated to detect genomic IGH-CCND1 fusion by polymerase chain reaction (PCR) using DNA extracted from frozen tissue. The IGH-CCND1 fusion detection rate in the MCLs was 96% by FISH compared with 35% by PCR. By FISH, one MCL and three small B-cell lymphomas other than MCL harboured abnormalities involving only IGH. Less than 1% of cells showed false-positive IGH-CCND1 fusion in normal specimens by FISH. Thus, this highly sensitive FISH assay is very useful in confirming the diagnosis of MCL, has wide applicability as it may be performed on both paraffin-embedded and fresh tissue, and may also facilitate detection of translocations involving these loci in tumours other than MCL.

Original languageEnglish (US)
Pages (from-to)856-862
Number of pages7
JournalBritish journal of haematology
Volume110
Issue number4
DOIs
StatePublished - 2000

Keywords

  • Cytogenetics
  • FISH
  • Interphase nuclei
  • Mantle-cell lymphoma
  • T(11;14)(q13;q32)

ASJC Scopus subject areas

  • Hematology

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  • Cite this

    Remstein, E. D., Kurtin, P. J., Buño, I., Bailey, R. J., Proffitt, J., Wyatt, W. A., Hanson, C. A., & Dewald, G. W. (2000). Diagnostic utility of fluorescence in situ hybridization in mantle-cell lymphoma. British journal of haematology, 110(4), 856-862. https://doi.org/10.1046/j.1365-2141.2000.02303.x