Diagnostic potential of Raman spectroscopy in Barrett's esophagus

Louis Michel Wong Kee Song, Andrea Molckovsky, Kenneth Ke Ning Wang, Lawrence J. Burgart, Brion Dolenko, Rajmund L. Somorjai, Brian C. Wilson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

33 Citations (Scopus)

Abstract

Patients with Barrett's esophagus (BE) undergo periodic endoscopie surveillance with random biopsies in an effort to detect dysplastic or early cancerous lesions. Surveillance may be enhanced by near-infrared Raman spectroscopy (NIRS), which has the potential to identify endoscopically-occult dysplastic lesions within the Barrett's segment and allow for targeted biopsies. The aim of this study was to assess the diagnostic performance of NIRS for identifying dysplastic lesions in BE in vivo. Raman spectra (P exc=70 mW; t=5 s) were collected from Barrett's mucosa at endoscopy using a custom-built NIRS system (λ exc=785 nm) equipped with a filtered fiber-optic probe. Each probed site was biopsied for matching histological diagnosis as assessed by an expert pathologist. Diagnostic algorithms were developed using genetic algorithm-based feature selection and linear discriminant analysis, and classification was performed on all spectra with a bootstrap-based cross-validation scheme. The analysis comprised 192 samples (112 non-dysplastic, 54 low-grade dysplasia and 26 high-grade dysplasia/early adenocarcinoma) from 65 patients. Compared with histology, NIRS differentiated dysplastic from non-dysplastic Barrett's samples with 86% sensitivity, 88% specificity and 87% accuracy. NIRS identified 'high-risk' lesions (high-grade dysplasia/early adenocarcinoma) with 88% sensitivity, 89% specificity and 89% accuracy. In the present study, NIRS classified Barrett's epithelia with high and clinically-useful diagnostic accuracy.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
EditorsT. Vo-Dinh, W.S. Grundfest, D.A. Benaron, G.E. Cohn
Pages140-146
Number of pages7
Volume5692
DOIs
StatePublished - 2005
EventAdvanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States
Duration: Jan 23 2005Jan 26 2005

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems III
CountryUnited States
CitySan Jose, CA
Period1/23/051/26/05

Fingerprint

Raman spectroscopy
Infrared spectroscopy
Biopsy
Histology
Endoscopy
Discriminant analysis
Fiber optics
Raman scattering
Feature extraction
Genetic algorithms

Keywords

  • Barrett's esophagus
  • Dysplasia
  • Endoscopy
  • Raman spectroscopy

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Wong Kee Song, L. M., Molckovsky, A., Wang, K. K. N., Burgart, L. J., Dolenko, B., Somorjai, R. L., & Wilson, B. C. (2005). Diagnostic potential of Raman spectroscopy in Barrett's esophagus. In T. Vo-Dinh, W. S. Grundfest, D. A. Benaron, & G. E. Cohn (Eds.), Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 5692, pp. 140-146). [23] https://doi.org/10.1117/12.584986

Diagnostic potential of Raman spectroscopy in Barrett's esophagus. / Wong Kee Song, Louis Michel; Molckovsky, Andrea; Wang, Kenneth Ke Ning; Burgart, Lawrence J.; Dolenko, Brion; Somorjai, Rajmund L.; Wilson, Brian C.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. ed. / T. Vo-Dinh; W.S. Grundfest; D.A. Benaron; G.E. Cohn. Vol. 5692 2005. p. 140-146 23.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wong Kee Song, LM, Molckovsky, A, Wang, KKN, Burgart, LJ, Dolenko, B, Somorjai, RL & Wilson, BC 2005, Diagnostic potential of Raman spectroscopy in Barrett's esophagus. in T Vo-Dinh, WS Grundfest, DA Benaron & GE Cohn (eds), Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 5692, 23, pp. 140-146, Advanced Biomedical and Clinical Diagnostic Systems III, San Jose, CA, United States, 1/23/05. https://doi.org/10.1117/12.584986
Wong Kee Song LM, Molckovsky A, Wang KKN, Burgart LJ, Dolenko B, Somorjai RL et al. Diagnostic potential of Raman spectroscopy in Barrett's esophagus. In Vo-Dinh T, Grundfest WS, Benaron DA, Cohn GE, editors, Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 5692. 2005. p. 140-146. 23 https://doi.org/10.1117/12.584986
Wong Kee Song, Louis Michel ; Molckovsky, Andrea ; Wang, Kenneth Ke Ning ; Burgart, Lawrence J. ; Dolenko, Brion ; Somorjai, Rajmund L. ; Wilson, Brian C. / Diagnostic potential of Raman spectroscopy in Barrett's esophagus. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. editor / T. Vo-Dinh ; W.S. Grundfest ; D.A. Benaron ; G.E. Cohn. Vol. 5692 2005. pp. 140-146
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