Diagnostic potential of Raman spectroscopy in Barrett's esophagus

Louis Michel Wong Kee Song, Andrea Molckovsky, Kenneth K. Wang, Lawrence J. Burgart, Brion Dolenko, Rajmund L. Somorjai, Brian C. Wilson

Research output: Contribution to journalConference articlepeer-review

35 Scopus citations


Patients with Barrett's esophagus (BE) undergo periodic endoscopie surveillance with random biopsies in an effort to detect dysplastic or early cancerous lesions. Surveillance may be enhanced by near-infrared Raman spectroscopy (NIRS), which has the potential to identify endoscopically-occult dysplastic lesions within the Barrett's segment and allow for targeted biopsies. The aim of this study was to assess the diagnostic performance of NIRS for identifying dysplastic lesions in BE in vivo. Raman spectra (Pexc=70 mW; t=5 s) were collected from Barrett's mucosa at endoscopy using a custom-built NIRS system (λexc=785 nm) equipped with a filtered fiber-optic probe. Each probed site was biopsied for matching histological diagnosis as assessed by an expert pathologist. Diagnostic algorithms were developed using genetic algorithm-based feature selection and linear discriminant analysis, and classification was performed on all spectra with a bootstrap-based cross-validation scheme. The analysis comprised 192 samples (112 non-dysplastic, 54 low-grade dysplasia and 26 high-grade dysplasia/early adenocarcinoma) from 65 patients. Compared with histology, NIRS differentiated dysplastic from non-dysplastic Barrett's samples with 86% sensitivity, 88% specificity and 87% accuracy. NIRS identified 'high-risk' lesions (high-grade dysplasia/early adenocarcinoma) with 88% sensitivity, 89% specificity and 89% accuracy. In the present study, NIRS classified Barrett's epithelia with high and clinically-useful diagnostic accuracy.

Original languageEnglish (US)
Article number23
Pages (from-to)140-146
Number of pages7
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
StatePublished - 2005
EventAdvanced Biomedical and Clinical Diagnostic Systems III - San Jose, CA, United States
Duration: Jan 23 2005Jan 26 2005


  • Barrett's esophagus
  • Dysplasia
  • Endoscopy
  • Raman spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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