Diagnostic performance of two confocal endomicroscopy systems in detecting Barrett's dysplasia: A pilot study using a novel bioprobe in ex vivo tissue

Emmanuel C. Gorospe, Cadman L. Leggett, Gang Sun, Marlys A. Anderson, Milli Gupta, Joshua D. Penfield, Lori Lutzke, Jason T. Lewis, Louis M. Wong Kee Song, Kenneth K. Wang

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Background: There are currently 2 existing confocal laser endomicroscopy (CLE) platforms: probe-based CLE (pCLE) and endoscope-based CLE (eCLE) systems, each with its own criteria for identifying dysplasia in Barrett's esophagus (BE). The diagnostic performance of these 2 systems has not been directly compared. Design: Preclinical, feasibility study. Objectives: We compared the interrater agreement and diagnostic performance of the pCLE and eCLE systems. In addition, we evaluated a new BE endomicroscopy criteria based on fluorescent glucose intensity uptake. Patients: Thirteen patients with Barrett's esophagus and high-grade dysplasia or early cancer undergoing 16 EMR. Intervention: CLE imaging was performed using two different probes with 2-[N-(7-nitrobenz-2-oxa-1, 3-diaxol-4-yl)amino]-2-deoxyglucose, a fluorescent glucose analog with preferential uptake in dysplastic mucosa to supply contrast. Four quadrants were imaged per specimen with a total of 64 imaged mucosal sites presented to three gastroenterologists. Main Outcome Measurements: Interobserver agreement and accuracy for dysplasia was assessed of images classified according to Miami criteria, stacked eCLE images classified using the Mainz criteria and a novel fluorescence intensity criteria. Results: The interrater agreements were 0.17, 0.68, and 0.87 for the Miami, Mainz, and the fluorescence intensity criteria, respectively. Overall accuracy in detecting dysplasia was 37% (95% CI, 30.3-43.9), 44.3% (95% CI, 37.3-50.9), and 78.6% (95% CI, 72.2-83.3) for the Miami, Mainz, and the fluorescence intensity criteria, respectively. Limitations: This imaging technique and proposed fluorescence intensity criteria using 2-[N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose in EMR tissue will require in vivo validation and cannot be directly used with the current eCLE and pCLE clinical applications. Conclusions: In this preclinical feasibility study, the use of an eCLE system with a topical fluorescent contrast in ex vivo EMR tissue demonstrated higher interrater agreement and accuracy.

Original languageEnglish (US)
Pages (from-to)933-938
Number of pages6
JournalGastrointestinal endoscopy
Volume76
Issue number5
DOIs
StatePublished - Nov 2012

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Gastroenterology

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