Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal

Megan M. Garrity, Simon J. Gibbons, Thomas Christopher Smyrk, Jean Marie Vanderwinden, Pedro Julian Gomez-Pinilla, Anoop Nehra, Matthew Borg, Gianrico Farrugia

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Garrity M M, Gibbons S J, Smyrk T C, Vanderwinden J M, Gomez-Pinilla P J, Nehra A, Borg M & Farrugia G(2009) Histopathology 54, 286-294Diagnostic challenges of motility disorders: optimal detection of CD117+ interstitial cells of CajalAims: Several gastrointestinal motility diseases are associated with altered numbers of interstitial cells of Cajal (ICC), and testing for alterations in numbers of ICC has been proposed as one way to improve routine diagnosis in motility diseases. However, the protocols currently used to visualize ICC in formalin-fixed paraffin-embedded (FFPE) tissue using antibodies to CD117 have not been optimized for studying motility disorders. The aims of this study were therefore to determine the optimal protocol using FFPE tissue, determine normal values for ICC in non-neoplastic human colon, and compare results with those obtained using immunofluorescence (IF). Methods and results: Non-neoplastic tissue was collected from patients undergoing resection for colonic cancer and fixed for both light (FFPE) and IF testing. Sections were processed for standard immunohistochemistry using different primary antibodies in conjunction with variations in antigen retrieval [ethylenediamine tetraacetricacid (EDTA), citrate], antibody dilution, blocking and detection (Mach2, Mach3, Envision+). Best results were obtained with EDTA retrieval, the DAKO CD117 antibody and Mach3 detection. Conclusions: The optimized protocol presented improved CD117 detection in FFPE tissues and showed good concordance with overall localization of CD117-immunoreactive ICC as detected by IF. As such, this protocol may be more useful than current diagnostic procedures in motility disorders.

Original languageEnglish (US)
Pages (from-to)286-294
Number of pages9
JournalHistopathology
Volume54
Issue number3
DOIs
StatePublished - Feb 2009

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Interstitial Cells of Cajal
ethylenediamine
Paraffin
Formaldehyde
Fluorescent Antibody Technique
Antibodies
Hylobates
Antigenic Variation
Gastrointestinal Motility
Blocking Antibodies
Gastrointestinal Diseases
Citric Acid
Colonic Neoplasms
Colon
Reference Values
Immunohistochemistry
Light

Keywords

  • C-Kit
  • Colon
  • Human
  • Immunofluorescence
  • Motility disorders

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Garrity, M. M., Gibbons, S. J., Smyrk, T. C., Vanderwinden, J. M., Gomez-Pinilla, P. J., Nehra, A., ... Farrugia, G. (2009). Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal. Histopathology, 54(3), 286-294. https://doi.org/10.1111/j.1365-2559.2008.03189.x

Diagnostic challenges of motility disorders : Optimal detection of CD117+ interstitial cells of Cajal. / Garrity, Megan M.; Gibbons, Simon J.; Smyrk, Thomas Christopher; Vanderwinden, Jean Marie; Gomez-Pinilla, Pedro Julian; Nehra, Anoop; Borg, Matthew; Farrugia, Gianrico.

In: Histopathology, Vol. 54, No. 3, 02.2009, p. 286-294.

Research output: Contribution to journalArticle

Garrity, MM, Gibbons, SJ, Smyrk, TC, Vanderwinden, JM, Gomez-Pinilla, PJ, Nehra, A, Borg, M & Farrugia, G 2009, 'Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal', Histopathology, vol. 54, no. 3, pp. 286-294. https://doi.org/10.1111/j.1365-2559.2008.03189.x
Garrity MM, Gibbons SJ, Smyrk TC, Vanderwinden JM, Gomez-Pinilla PJ, Nehra A et al. Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal. Histopathology. 2009 Feb;54(3):286-294. https://doi.org/10.1111/j.1365-2559.2008.03189.x
Garrity, Megan M. ; Gibbons, Simon J. ; Smyrk, Thomas Christopher ; Vanderwinden, Jean Marie ; Gomez-Pinilla, Pedro Julian ; Nehra, Anoop ; Borg, Matthew ; Farrugia, Gianrico. / Diagnostic challenges of motility disorders : Optimal detection of CD117+ interstitial cells of Cajal. In: Histopathology. 2009 ; Vol. 54, No. 3. pp. 286-294.
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