Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal

Megan M. Garrity, Simon J. Gibbons, Thomas C. Smyrk, Jean Marie Vanderwinden, Pedro Julian Gomez-Pinilla, Anoop Nehra, Matthew Borg, Gianrico Farrugia

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Garrity M M, Gibbons S J, Smyrk T C, Vanderwinden J M, Gomez-Pinilla P J, Nehra A, Borg M & Farrugia G(2009) Histopathology 54, 286-294Diagnostic challenges of motility disorders: optimal detection of CD117+ interstitial cells of CajalAims: Several gastrointestinal motility diseases are associated with altered numbers of interstitial cells of Cajal (ICC), and testing for alterations in numbers of ICC has been proposed as one way to improve routine diagnosis in motility diseases. However, the protocols currently used to visualize ICC in formalin-fixed paraffin-embedded (FFPE) tissue using antibodies to CD117 have not been optimized for studying motility disorders. The aims of this study were therefore to determine the optimal protocol using FFPE tissue, determine normal values for ICC in non-neoplastic human colon, and compare results with those obtained using immunofluorescence (IF). Methods and results: Non-neoplastic tissue was collected from patients undergoing resection for colonic cancer and fixed for both light (FFPE) and IF testing. Sections were processed for standard immunohistochemistry using different primary antibodies in conjunction with variations in antigen retrieval [ethylenediamine tetraacetricacid (EDTA), citrate], antibody dilution, blocking and detection (Mach2, Mach3, Envision+). Best results were obtained with EDTA retrieval, the DAKO CD117 antibody and Mach3 detection. Conclusions: The optimized protocol presented improved CD117 detection in FFPE tissues and showed good concordance with overall localization of CD117-immunoreactive ICC as detected by IF. As such, this protocol may be more useful than current diagnostic procedures in motility disorders.

Original languageEnglish (US)
Pages (from-to)286-294
Number of pages9
JournalHistopathology
Volume54
Issue number3
DOIs
StatePublished - Feb 2009

Keywords

  • C-Kit
  • Colon
  • Human
  • Immunofluorescence
  • Motility disorders

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Fingerprint Dive into the research topics of 'Diagnostic challenges of motility disorders: Optimal detection of CD117+ interstitial cells of Cajal'. Together they form a unique fingerprint.

Cite this