Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

Mark J. Espy; Carlos V. Paya; Keith E. Holley; Jurgen Ludwig; Paul F. Hermans; Russell H. Wiesner; Ruud A.F. Krom; Thomas F. Smith

doi:10.1016/0732-8893(91)90019-C

Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

Mark J. Espy, Carlos V. Paya, Keith E. Holley, Jurgen Ludwig, Paul F. Hermans, Russell H. Wiesner, Ruud A.F. Krom, Thomas F. Smith

Gastroenterology and Hepatology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

Original language	English (US)
Pages (from-to)	293-296
Number of pages	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume	14
Issue number	4
DOIs	https://doi.org/10.1016/0732-8893(91)90019-C
State	Published - 1991

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1016/0732-8893(91)90019-C

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title = "Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation",

abstract = "Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.",

author = "Espy, {Mark J.} and Paya, {Carlos V.} and Holley, {Keith E.} and Jurgen Ludwig and Hermans, {Paul F.} and Wiesner, {Russell H.} and Krom, {Ruud A.F.} and Smith, {Thomas F.}",

year = "1991",

doi = "10.1016/0732-8893(91)90019-C",

language = "English (US)",

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T1 - Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

AU - Espy, Mark J.

AU - Paya, Carlos V.

AU - Holley, Keith E.

AU - Ludwig, Jurgen

AU - Hermans, Paul F.

AU - Wiesner, Russell H.

AU - Krom, Ruud A.F.

AU - Smith, Thomas F.

PY - 1991

Y1 - 1991

N2 - Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

AB - Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

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AN - SCOPUS:0025835596

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Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

Abstract

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