Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

Mark J. Espy, Carlos V. Paya, Keith E. Holley, Jurgen Ludwig, Paul F. Hermans, Russell H. Wiesner, Ruud A F Krom, Thomas F. Smith

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume14
Issue number4
DOIs
StatePublished - 1991

Fingerprint

Cytomegalovirus
Liver Transplantation
Hepatitis
In Situ Hybridization
DNA Probes
Inclusion Bodies
Hematoxylin
Eosine Yellowish-(YS)
Microbiology
Cell Culture Techniques
Staining and Labeling
Biopsy

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

Cite this

Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation. / Espy, Mark J.; Paya, Carlos V.; Holley, Keith E.; Ludwig, Jurgen; Hermans, Paul F.; Wiesner, Russell H.; Krom, Ruud A F; Smith, Thomas F.

In: Diagnostic Microbiology and Infectious Disease, Vol. 14, No. 4, 1991, p. 293-296.

Research output: Contribution to journalArticle

Espy, MJ, Paya, CV, Holley, KE, Ludwig, J, Hermans, PF, Wiesner, RH, Krom, RAF & Smith, TF 1991, 'Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation', Diagnostic Microbiology and Infectious Disease, vol. 14, no. 4, pp. 293-296. https://doi.org/10.1016/0732-8893(91)90019-C
Espy, Mark J. ; Paya, Carlos V. ; Holley, Keith E. ; Ludwig, Jurgen ; Hermans, Paul F. ; Wiesner, Russell H. ; Krom, Ruud A F ; Smith, Thomas F. / Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation. In: Diagnostic Microbiology and Infectious Disease. 1991 ; Vol. 14, No. 4. pp. 293-296.
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