Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation

Mark J. Espy, Carlos V. Paya, Keith E. Holley, Jurgen Ludwig, Paul F. Hermans, Russell H. Wiesner, Ruud A.F. Krom, Thomas F. Smith

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Hematoxylin-eosin staining (H&E) of cytomegalovirus (CMV) inclusion bodies was compared with in situ hybridization using a biotinylated DNA probe for the detection of CMV. Of 29 biopsy specimens selected from 23 patients with clinical CMV hepatitis and typical CMV inclusions on histopathologic examination, 23 (79%) were positive by DNA probe and 17 (59%) were detected in cell cultures. The mean number of CMV foci per tissue section was higher by DNA probe (12) compared with H&E (5). We do not recommend in situ hybridization in microbiology laboratories as a replacement for histopathology for the diagnosis of CMV in tissue specimens.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume14
Issue number4
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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    Espy, M. J., Paya, C. V., Holley, K. E., Ludwig, J., Hermans, P. F., Wiesner, R. H., Krom, R. A. F., & Smith, T. F. (1991). Diagnosis of cytomegalovirus hepatitis by histopathology and in situ hybridization in liver transplantation. Diagnostic Microbiology and Infectious Disease, 14(4), 293-296. https://doi.org/10.1016/0732-8893(91)90019-C