Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats

Paulo Emilio Botura Ferreira, Cláudia Regina Pinheiro Lopes, Angela Maria Pereira Alves, Éder Paulo Belato Alves, David R Linden, Jacqueline Nelisis Zanoni, Nilza Cristina Buttow

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.

Original languageEnglish (US)
Pages (from-to)6416-6426
Number of pages11
JournalWorld Journal of Gastroenterology
Volume19
Issue number38
DOIs
StatePublished - Oct 14 2013

Fingerprint

Cecum
Diabetic Neuropathies
Quercetin
Population
Nitric Oxide Synthase
Neuroglia
Neurons
Glycogen Synthase
Neuroprotective Agents
Population Density
Body Weight
Staining and Labeling
Control Groups

Keywords

  • Diabetes
  • Enteric glia
  • Myenteric plexus
  • Neuronal nitric oxide synthase
  • Neuroprotection
  • Vasoactive intestinal polypeptide

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Ferreira, P. E. B., Lopes, C. R. P., Alves, A. M. P., Alves, É. P. B., Linden, D. R., Zanoni, J. N., & Buttow, N. C. (2013). Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats. World Journal of Gastroenterology, 19(38), 6416-6426. https://doi.org/10.3748/wjg.v19.i38.6416

Diabetic neuropathy : An evaluation of the use of quercetin in the cecum of rats. / Ferreira, Paulo Emilio Botura; Lopes, Cláudia Regina Pinheiro; Alves, Angela Maria Pereira; Alves, Éder Paulo Belato; Linden, David R; Zanoni, Jacqueline Nelisis; Buttow, Nilza Cristina.

In: World Journal of Gastroenterology, Vol. 19, No. 38, 14.10.2013, p. 6416-6426.

Research output: Contribution to journalArticle

Ferreira, PEB, Lopes, CRP, Alves, AMP, Alves, ÉPB, Linden, DR, Zanoni, JN & Buttow, NC 2013, 'Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats', World Journal of Gastroenterology, vol. 19, no. 38, pp. 6416-6426. https://doi.org/10.3748/wjg.v19.i38.6416
Ferreira, Paulo Emilio Botura ; Lopes, Cláudia Regina Pinheiro ; Alves, Angela Maria Pereira ; Alves, Éder Paulo Belato ; Linden, David R ; Zanoni, Jacqueline Nelisis ; Buttow, Nilza Cristina. / Diabetic neuropathy : An evaluation of the use of quercetin in the cecum of rats. In: World Journal of Gastroenterology. 2013 ; Vol. 19, No. 38. pp. 6416-6426.
@article{1c0cee72af9047a4b00d33fe17393fd5,
title = "Diabetic neuropathy: An evaluation of the use of quercetin in the cecum of rats",
abstract = "Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25{\%}) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.",
keywords = "Diabetes, Enteric glia, Myenteric plexus, Neuronal nitric oxide synthase, Neuroprotection, Vasoactive intestinal polypeptide",
author = "Ferreira, {Paulo Emilio Botura} and Lopes, {Cl{\'a}udia Regina Pinheiro} and Alves, {Angela Maria Pereira} and Alves, {{\'E}der Paulo Belato} and Linden, {David R} and Zanoni, {Jacqueline Nelisis} and Buttow, {Nilza Cristina}",
year = "2013",
month = "10",
day = "14",
doi = "10.3748/wjg.v19.i38.6416",
language = "English (US)",
volume = "19",
pages = "6416--6426",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "38",

}

TY - JOUR

T1 - Diabetic neuropathy

T2 - An evaluation of the use of quercetin in the cecum of rats

AU - Ferreira, Paulo Emilio Botura

AU - Lopes, Cláudia Regina Pinheiro

AU - Alves, Angela Maria Pereira

AU - Alves, Éder Paulo Belato

AU - Linden, David R

AU - Zanoni, Jacqueline Nelisis

AU - Buttow, Nilza Cristina

PY - 2013/10/14

Y1 - 2013/10/14

N2 - Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.

AB - Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.

KW - Diabetes

KW - Enteric glia

KW - Myenteric plexus

KW - Neuronal nitric oxide synthase

KW - Neuroprotection

KW - Vasoactive intestinal polypeptide

UR - http://www.scopus.com/inward/record.url?scp=84885646002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885646002&partnerID=8YFLogxK

U2 - 10.3748/wjg.v19.i38.6416

DO - 10.3748/wjg.v19.i38.6416

M3 - Article

C2 - 24151360

AN - SCOPUS:84885646002

VL - 19

SP - 6416

EP - 6426

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 38

ER -