TY - JOUR
T1 - Diabetic neuropathy
T2 - An evaluation of the use of quercetin in the cecum of rats
AU - Ferreira, Paulo Emilio Botura
AU - Lopes, Cláudia Regina Pinheiro
AU - Alves, Angela Maria Pereira
AU - Alves, Éder Paulo Belato
AU - Linden, David Robert
AU - Zanoni, Jacqueline Nelisis
AU - Buttow, Nilza Cristina
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2013/10/14
Y1 - 2013/10/14
N2 - Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.
AB - Aim: To investigate the effect of quercetin supplementation on the myenteric neurons and glia in the cecum of diabetic rats. Methods: Total preparations of the muscular tunic were prepared from the ceca of twenty-four rats divided into the following groups: control (C), control supplemented with quercetin (200 mg/kg quercetin body weight) (CQ), diabetic (D) and diabetic supplemented with quercetin (DQ). Immunohistochemical double staining technique was performed with HuC/D (general population)/nitric oxide synthase (nNOS), HuC-D/S-100 and VIP. Density analysis of the general neuronal population HuC/D-IR, the nNOS-IR (nitrergic subpopulation) and the enteric glial cells (S-100) was performed, and the morphometry and the reduction in varicosity population (VIP-IR) in these populations were analyzed. Results: Diabetes promoted a significant reduction (25%) in the neuronal density of the HuC/D-IR (general population) and the nNOS-IR (nitrergic subpopulation) compared with the C group. Diabetes also significantly increased the areas of neurons, glial cells and VIP-IR varicosities. Supplementation with quercetin in the DQ group prevented neuronal loss in the general population and increased its area (P < 0.001) and the area of nitrergic subpopulation (P < 0.001), when compared to C group. Quercetin induced a VIP-IR and glial cells areas (P < 0.001) in DQ group when compared to C, CQ and D groups. Conclusion: In diabetes, quercetin exhibited a neuroprotective effect by maintaining the density of the general neuronal population but did not affect the density of the nNOS subpopulation.
KW - Diabetes
KW - Enteric glia
KW - Myenteric plexus
KW - Neuronal nitric oxide synthase
KW - Neuroprotection
KW - Vasoactive intestinal polypeptide
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U2 - 10.3748/wjg.v19.i38.6416
DO - 10.3748/wjg.v19.i38.6416
M3 - Article
C2 - 24151360
AN - SCOPUS:84885646002
SN - 1007-9327
VL - 19
SP - 6416
EP - 6426
JO - World journal of gastroenterology
JF - World journal of gastroenterology
IS - 38
ER -