Dexamethasone and lidocaine suppress eosinophilopoiesis from umbilical cord blood cells

Masato Muraki, Hirohito Kita, Gerald J. Gleich

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Eosinophils play an important role in allergic inflammation. Glucocorticosteroids have been used as an anti-inflammatory medication for inflammatory diseases involving eosinophil infiltration. Some effect of nebulized lidocaine has been reported when treating certain patients with asthma, which is also an inflammatory disease. The goal of this study was to examine the effects of dexamethasone and lidocaine on eosinophil proliferation and differentiation using a model of human umbilical cord blood mononuclear cells (UCMC) cultured with IL-5. Methods: UCMC were cultured with IL-5 (5 ng/mL) for 4 weeks. The effects of dexamethasone and lidocaine on the number and morphology of eosinophilic cells were visualized with Wright-Giemsa and cyanide-resistant peroxidase stains. Moreover, the effect on eosinophil-derived neurotoxin (EDN) and eosinophil peroxidase (EPX) contents in cultured cells were evaluated using radioimmunoassay. Results: The number of eosinophilic cells and EDN and EPX content in cultured cells increased in a time-dependent manner in the presence of IL-5. Dexamethasone treatment slightly decreased the number of eosinophilic cells in one week, but this effect was lost in 2–4 weeks. Macrophages in cultured UCMC treated with dexamethasone contained more eosinophil granule proteins. Both EDN and EPX content in cultured cells were reduced by dexamethasone. Lidocaine decreased the number of eosinophilic cells and reduced both EDN and EPX contents in cultured cells. Conclusions: Dexamethasone suppressed the production of eosinophil granule proteins and may also induce apoptosis of eosinophils, while lidocaine suppresses eosinophilopoiesis.

Original languageEnglish (US)
Article number24
JournalClinical and Molecular Allergy
Volume18
Issue number1
DOIs
StatePublished - Dec 2020

Keywords

  • Dexamethasone
  • Eosinophil peroxidase
  • Eosinophil-derived neurotoxin
  • Eosinophilopoiesis
  • Lidocaine
  • Umbilical cord blood

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Molecular Biology

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