Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus

Kyoko Kurosawa; Yasuhiro Ikeda; Takayuki Miyazawa; Yoshihiro Izumiya; Yorihiro Nishimura; Kazuya Nakamura; Eiji Sato; Takeshi Mikami; Chieko Kai; Eiji Takahashi

doi:10.1111/j.1348-0421.1999.tb02476.x

Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus

Kyoko Kurosawa, Yasuhiro Ikeda, Takayuki Miyazawa, Yoshihiro Izumiya, Yorihiro Nishimura, Kazuya Nakamura, Eiji Sato, Takeshi Mikami, Chieko Kai, Eiji Takahashi

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.

Original language	English (US)
Pages (from-to)	817-820
Number of pages	4
Journal	Microbiology and Immunology
Volume	43
Issue number	8
DOIs	https://doi.org/10.1111/j.1348-0421.1999.tb02476.x
State	Published - 1999

Keywords

Feline immunodeficiency virus
Polymerase chain reaction
Restriction fragment-length polymorphism

ASJC Scopus subject areas

Microbiology
Immunology
Virology

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10.1111/j.1348-0421.1999.tb02476.x

Cite this

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abstract = "Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.",

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author = "Kyoko Kurosawa and Yasuhiro Ikeda and Takayuki Miyazawa and Yoshihiro Izumiya and Yorihiro Nishimura and Kazuya Nakamura and Eiji Sato and Takeshi Mikami and Chieko Kai and Eiji Takahashi",

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T1 - Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus

AU - Kurosawa, Kyoko

AU - Ikeda, Yasuhiro

AU - Miyazawa, Takayuki

AU - Izumiya, Yoshihiro

AU - Nishimura, Yorihiro

AU - Nakamura, Kazuya

AU - Sato, Eiji

AU - Mikami, Takeshi

AU - Kai, Chieko

AU - Takahashi, Eiji

PY - 1999

Y1 - 1999

N2 - Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.

AB - Feline immunodeficiency virus (FIV) isolates have been classified into five subtypes (A to E) based on the sequences of the env variable V3 to V5 region. In this study, we sequenced a partial gag region of 4 and 3 isolates belonging to subtypes C and E, respectively. Phylogenetic analysis revealed that the branching pattern based on the region was similar to that based on the env V3 to V5 region. Here, we propose a protocol to differentiate five subtypes by polymerase chain reaction amplifying 329 bp within the region followed by restriction fragment-length polymorphism analysis using four restriction enzymes.

KW - Feline immunodeficiency virus

KW - Polymerase chain reaction

KW - Restriction fragment-length polymorphism

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Development of restriction fragment-length polymorphism method to differentiate five subtypes of feline immunodeficiency virus

Abstract

Keywords

ASJC Scopus subject areas

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