Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate)

Richard G. Payne, Joseph S. McGonigle, Michael J Yaszemski, Alan W. Yasko, Antonios G. Mikos

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.

Original languageEnglish (US)
Pages (from-to)4373-4380
Number of pages8
JournalBiomaterials
Volume23
Issue number22
DOIs
StatePublished - 2002

Fingerprint

Osteoblasts
Fumarates
Crosslinking
Polypropylenes
Propylene
Bone Marrow
Cells
Injections
Composite materials
Cell Survival
Population
Gelatin
Encapsulation
Cell Proliferation
Tissue culture
Polystyrenes
poly(propylene fumarate)
Scaffolds
Thymidine
propylene

Keywords

  • Bone tissue engineering
  • Cell transplantation
  • Gelatin microparticles
  • Injectable biomaterials
  • Marrow stromal osteoblasts
  • Poly(propylene fumarate)
  • Temporary cell encapsulation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

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title = "Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate)",
abstract = "The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.",
keywords = "Bone tissue engineering, Cell transplantation, Gelatin microparticles, Injectable biomaterials, Marrow stromal osteoblasts, Poly(propylene fumarate), Temporary cell encapsulation",
author = "Payne, {Richard G.} and McGonigle, {Joseph S.} and Yaszemski, {Michael J} and Yasko, {Alan W.} and Mikos, {Antonios G.}",
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T1 - Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate)

AU - Payne, Richard G.

AU - McGonigle, Joseph S.

AU - Yaszemski, Michael J

AU - Yasko, Alan W.

AU - Mikos, Antonios G.

PY - 2002

Y1 - 2002

N2 - The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.

AB - The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.

KW - Bone tissue engineering

KW - Cell transplantation

KW - Gelatin microparticles

KW - Injectable biomaterials

KW - Marrow stromal osteoblasts

KW - Poly(propylene fumarate)

KW - Temporary cell encapsulation

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