TY - JOUR
T1 - Development of an injectable, in situ crosslinkable, degradable polymeric carrier for osteogenic cell populations. Part 2. Viability of encapsulated marrow stromal osteoblasts cultured on crosslinking poly(propylene fumarate)
AU - Payne, Richard G.
AU - McGonigle, Joseph S.
AU - Yaszemski, Michael J.
AU - Yasko, Alan W.
AU - Mikos, Antonios G.
N1 - Funding Information:
This work was supported by the National Institutes of Health (R01-DE13031). Richard G. Payne acknowledges financial support by the National Institutes of Health Biotechnology Training Grant (5T32GM08362).
PY - 2002
Y1 - 2002
N2 - The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.
AB - The effect of temporary encapsulation of rat marrow stromal osteoblasts in crosslinked gelatin microparticles on cell viability and proliferation was investigated in this study for microparticles placed on a crosslinking poly(propylene fumarate) (PPF) composite over a 7 day time period. Encapsulated cells were seeded on crosslinking PPF composites at times up to 10min following initiation of the crosslinking reaction, and also on fully crosslinked PPF composites and tissue culture polystyrene controls, with a cell seeding density of 5.3×104 cells/cm2. The crosslinked PPF composite exhibited an average gel point of 10.3min and an average maximum crosslinking temperature of 47.5°C. Cell viability and proliferation were assessed by DNA and 3H-thymidine assays and the results were compared with those for nonencapsulated cells. The results showed that the addition time of cells to a crosslinking PPF composite had a large effect on cell viability and proliferation for both encapsulated and nonencapsulated cells with more surviving cells added at later time points. Most importantly, the temporary encapsulation of cells significantly enhanced cell viability at earlier time points. The data indicate that the presence of gelatin microparticles does not affect the crosslinking of a PPF composite. They further suggest that the temporary encapsulation of cells in crosslinked gelatin microparticles may preserve the viability of cells contained in an actively crosslinking PPF composite used as an injectable polymeric scaffold serving also as a carrier for osteogenic cell populations.
KW - Bone tissue engineering
KW - Cell transplantation
KW - Gelatin microparticles
KW - Injectable biomaterials
KW - Marrow stromal osteoblasts
KW - Poly(propylene fumarate)
KW - Temporary cell encapsulation
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U2 - 10.1016/S0142-9612(02)00185-0
DO - 10.1016/S0142-9612(02)00185-0
M3 - Article
C2 - 12219827
AN - SCOPUS:0036345612
SN - 0142-9612
VL - 23
SP - 4373
EP - 4380
JO - Biomaterials
JF - Biomaterials
IS - 22
ER -