Development and validation of a new radioimmunoassay for parathyrin (PTH)

P. C. Kao, N. S. Jiang, G. G. Klee, D. C. Purnell

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

A radioimmunoassay for human parathyrin has been developed and characterized with whole-molecule (residues 1-84) human parathyrin and with the 1-34, 44-68, and 53-84 amino acid residue fragments of it. The antiserum used reacted with the whole molecule and with the 44-68 and 53-84 fragments, but not with the 1-34 fragment. Parathyrin concentrations in the serum of 118 normal subjects and of 112 patients with surgically proved primary hyperparathyroidism were determined with this assay. The mean results were 39 (SD 13) μL-Eq/mL for the normals and 111 (SD 77) μL-Eq/mL for the patients with primary hyperparathyroidism (p < 0.0005). The upper 95% confidence limit of the normal range was 60 μL-Eq/mL. For 54 patients with primary hyperparathyroidism, the preoperative values for serum parathyrin, calcium, and phosphate - but not creatine - were statistically different from the postoperative values (paired t-test, p < 0.0005). Normal subjects showed significant (p < 0.0005) differences in serum calcium concentrations but not in parathyrin concentrations, compared with concentrations found in cancer patients and patients who had thiazide-induced hypercalcemia. Phosphate concentration in serum, although not a specific indicator of disease, is a valuable clue to the diagnosis of primary hyperparathyroidism.

Original languageEnglish (US)
Pages (from-to)69-74
Number of pages6
JournalClinical chemistry
Volume28
Issue number1
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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