Development and validation of a nanometer manipulation and measurement system for biomechanical testing of single macro-molecules

Zong Ping Luo, Kai Nan An

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A biomechanical testing system for single macro-molecules has been developed using optical tweezers. To achieve nanometer manipulation and measurement, a piezo-stage and an interferometer were adopted. A systematic calibration was then performed on the displacement resolution. The piezo- stage has a noise level around 1 nm standard deviation, and the interferometer has a noise level of less than 5 nm standard deviation. The results revealed that the system has a nanometer resolution which is sufficient for the measurement of deformation and stiffness of single macro- molecules.

Original languageEnglish (US)
Pages (from-to)1075-1079
Number of pages5
JournalJournal of Biomechanics
Volume31
Issue number11
DOIs
StatePublished - Nov 1 1998

Keywords

  • Interferometer
  • Molecular biomechanics
  • Nanometer
  • Optical tweezers
  • Piezo- stage

ASJC Scopus subject areas

  • Biophysics
  • Orthopedics and Sports Medicine
  • Biomedical Engineering
  • Rehabilitation

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