TY - JOUR
T1 - Development and preliminary testing of a translational model of hepatocellular carcinoma for MR imaging and interventional oncologic investigations
AU - Thompson, Scott M.
AU - Callstrom, Matthew R.
AU - Knudsen, Bruce
AU - Anderson, Jill L.
AU - Carter, Rickey E.
AU - Grande, Joseph P.
AU - Roberts, Lewis R.
AU - Woodrum, David A.
N1 - Funding Information:
Infrastructure support was provided by National Institutes of Health (NIH) Construction Grant C06 RR018898 and Center for Translational Science Activities/NIH Grant UL1 RR024150 . Research support was provided in part by a Society of Interventional Radiology (SIR) Foundation Allied Scientist Training Grant (to S.M.T.) and a Radiological Society of North America (RSNA) Research Scholar Grant (to D.A.W.). S.M.T. has received a research grant from the SIR Foundation ( SIR Allied Scientist Training Grant). D.A.W. has received a research grant from Visualase (Houston, Texas) and an RSNA Research Scholar Grant. M.R.C. has received research grants from Endocare (Irvine, California) and Siemens (Forchheim, Germany). None of the other authors have identified a conflict of interest.
PY - 2012/3
Y1 - 2012/3
N2 - Purpose: To develop a translational rat hepatocellular carcinoma (HCC) disease model for magnetic resonance (MR) imaging and image-guided interventional oncologic investigations. Materials and Methods: Male rats underwent sham control surgery (n = 6), selective bile duct ligation (SBDL; n = 4), or common bile duct ligation (CBDL; n = 6), with procedure optimization in four rats and N1S1 hepatoma cell injection into two or three sites in the livers of 12 rats. All rats subsequently underwent MR imaging to assess tumor establishment and volume. Mesenteric angiography and percutaneous MR-guided laser ablation of the liver were performed in a subgroup of animals (n = 4). Animal weight and liver test results were monitored. After harvesting, the livers were subjected to gross and microscopic analysis. Tumor volume and laboratory parameters were assessed between ligation groups. Results: MR imaging demonstrated hyperintense T2 and hypointense T1 lesions with tumor induction in five of 10 (50.0%), seven of eight (87.5%), and 12 of 12 (100%) sites in the control, SBDL, and CBDL groups, respectively. Tumor volumes differed significantly by group (P <.02). Mesenteric angiography demonstrated an enhancing tumor stain. Clinical and laboratory assessment revealed a significant decrease in weight (P =.01) and albumin level (P <.01) and an increase in total bilirubin level (P =.02) in CBDL rats but not SBDL rats (P = 1.0). Histologic examination showed high-grade HCCs with local and vascular invasion within the context of early fibrosis in CBDL and SBDL rats. MR-guided laser ablation generated a 12-cm ablation zone with histologic findings consistent with reversible and irreversible injury. Conclusions: A biologically relevant rat HCC disease model has been developed for MR imaging and preliminary interventional oncologic applications.
AB - Purpose: To develop a translational rat hepatocellular carcinoma (HCC) disease model for magnetic resonance (MR) imaging and image-guided interventional oncologic investigations. Materials and Methods: Male rats underwent sham control surgery (n = 6), selective bile duct ligation (SBDL; n = 4), or common bile duct ligation (CBDL; n = 6), with procedure optimization in four rats and N1S1 hepatoma cell injection into two or three sites in the livers of 12 rats. All rats subsequently underwent MR imaging to assess tumor establishment and volume. Mesenteric angiography and percutaneous MR-guided laser ablation of the liver were performed in a subgroup of animals (n = 4). Animal weight and liver test results were monitored. After harvesting, the livers were subjected to gross and microscopic analysis. Tumor volume and laboratory parameters were assessed between ligation groups. Results: MR imaging demonstrated hyperintense T2 and hypointense T1 lesions with tumor induction in five of 10 (50.0%), seven of eight (87.5%), and 12 of 12 (100%) sites in the control, SBDL, and CBDL groups, respectively. Tumor volumes differed significantly by group (P <.02). Mesenteric angiography demonstrated an enhancing tumor stain. Clinical and laboratory assessment revealed a significant decrease in weight (P =.01) and albumin level (P <.01) and an increase in total bilirubin level (P =.02) in CBDL rats but not SBDL rats (P = 1.0). Histologic examination showed high-grade HCCs with local and vascular invasion within the context of early fibrosis in CBDL and SBDL rats. MR-guided laser ablation generated a 12-cm ablation zone with histologic findings consistent with reversible and irreversible injury. Conclusions: A biologically relevant rat HCC disease model has been developed for MR imaging and preliminary interventional oncologic applications.
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U2 - 10.1016/j.jvir.2011.11.018
DO - 10.1016/j.jvir.2011.11.018
M3 - Article
C2 - 22265247
AN - SCOPUS:84862777362
SN - 1051-0443
VL - 23
SP - 385
EP - 395
JO - Journal of Vascular and Interventional Radiology
JF - Journal of Vascular and Interventional Radiology
IS - 3
ER -