Development and notch signaling requirements of the zebrafish choroid plexus

Brent R. Bill, Darius Balciunas, Joshua A. McCarra, Eric D. Young, Toua Xiong, Ashley M. Spahn, Marta Garcia-Lecea, Vladimir Korzh, Stephen C Ekker, Lisa A. Schimmenti

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: The choroid plexus (CP) is an epithelial and vascular structure in the ventricular system of the brain that is a critical part of the blood-brain barrier. The CP has two primary functions, 1) to produce and regulate components of the cerebral spinal fluid, and 2) to inhibit entry into the brain of exogenous substances. Despite its importance in neurobiology, little is known about how this structure forms. Methodology and Principal Findings: Here we show that the transposon-mediated enhancer trap zebrafish line EtMn16 expresses green fluorescent protein within a population of cells that migrate toward the midline and coalesce to form the deifinitive CP. We further demonstrate the development of the integral vascular network of the definitive CP. Utilizing pharmacologic pan-notch inhibition and specific morpholino-mediated knockdown, we demonstrate a requirement for Notch signaling in choroid plexus development. We identify three Notch signaling pathway members as mediating this effect, notch1b, deltaA and deltaD. Conclusions and Significance: This work is the first to identify the zebrafish choroid plexus and to characterize its epithelial and vasculatre integration. This study, in the context of other comparative anatomical studies, strongly indicates a conserved mechanism for development of the CP. Finally, we characterize a requirement for Notch signaling in the developing CP. This establishes the zebrafish CP as an important new system for the determination of key signaling pathways in the formation of this essential component of the vertebrate brain.

Original languageEnglish (US)
Article numbere3114
JournalPLoS One
Volume3
Issue number9
DOIs
StatePublished - Sep 3 2008

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choroid plexus
Choroid Plexus
Zebrafish
Danio rerio
Brain
Morpholinos
Green Fluorescent Proteins
brain
blood vessels
Blood Vessels
Cells
Fluids
enhancer elements
neurophysiology
blood-brain barrier
Neurobiology
Blood-Brain Barrier
green fluorescent protein
transposons
Vertebrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Bill, B. R., Balciunas, D., McCarra, J. A., Young, E. D., Xiong, T., Spahn, A. M., ... Schimmenti, L. A. (2008). Development and notch signaling requirements of the zebrafish choroid plexus. PLoS One, 3(9), [e3114]. https://doi.org/10.1371/journal.pone.0003114

Development and notch signaling requirements of the zebrafish choroid plexus. / Bill, Brent R.; Balciunas, Darius; McCarra, Joshua A.; Young, Eric D.; Xiong, Toua; Spahn, Ashley M.; Garcia-Lecea, Marta; Korzh, Vladimir; Ekker, Stephen C; Schimmenti, Lisa A.

In: PLoS One, Vol. 3, No. 9, e3114, 03.09.2008.

Research output: Contribution to journalArticle

Bill, BR, Balciunas, D, McCarra, JA, Young, ED, Xiong, T, Spahn, AM, Garcia-Lecea, M, Korzh, V, Ekker, SC & Schimmenti, LA 2008, 'Development and notch signaling requirements of the zebrafish choroid plexus', PLoS One, vol. 3, no. 9, e3114. https://doi.org/10.1371/journal.pone.0003114
Bill BR, Balciunas D, McCarra JA, Young ED, Xiong T, Spahn AM et al. Development and notch signaling requirements of the zebrafish choroid plexus. PLoS One. 2008 Sep 3;3(9). e3114. https://doi.org/10.1371/journal.pone.0003114
Bill, Brent R. ; Balciunas, Darius ; McCarra, Joshua A. ; Young, Eric D. ; Xiong, Toua ; Spahn, Ashley M. ; Garcia-Lecea, Marta ; Korzh, Vladimir ; Ekker, Stephen C ; Schimmenti, Lisa A. / Development and notch signaling requirements of the zebrafish choroid plexus. In: PLoS One. 2008 ; Vol. 3, No. 9.
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