Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute- Waldenström Macroglobulinemia 1

Kasyapa S. Chitta, Aneel Paulus, Sikander Ailawadhi, Barbara A. Foster, Michael T. Moser, Petr Starostik, Aisha Masood, Taimur Sher, Kena C. Miller, Dan M. Iancu, Jeffrey Conroy, Norma J. Nowak, Sheila N. Sait, David A. Personett, Morton Coleman, Richard R. Furman, Peter Martin, Stephen M. Ansell, Kelvin Lee, Asher A. Chanan-Khan

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Understanding the biology of Waldenström macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2), with a consistent amplification of 14q32 (immunoglobulin heavy chain; IgH) identical to its founding tumor sample. The clonal relationship is confirmed by identical CDR3 length and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in the MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Overall, RPCI-WM1 represents a valuable model to study Waldenström macroglobulinemia.

Original languageEnglish (US)
Pages (from-to)387-396
Number of pages10
JournalLeukemia and Lymphoma
Volume54
Issue number2
DOIs
StatePublished - Feb 1 2013

Keywords

  • Cell line
  • Model
  • Preclinical
  • Waldenström macroglobulinemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Chitta, K. S., Paulus, A., Ailawadhi, S., Foster, B. A., Moser, M. T., Starostik, P., Masood, A., Sher, T., Miller, K. C., Iancu, D. M., Conroy, J., Nowak, N. J., Sait, S. N., Personett, D. A., Coleman, M., Furman, R. R., Martin, P., Ansell, S. M., Lee, K., & Chanan-Khan, A. A. (2013). Development and characterization of a novel human Waldenström macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute- Waldenström Macroglobulinemia 1. Leukemia and Lymphoma, 54(2), 387-396. https://doi.org/10.3109/10428194.2012.713481