@article{84edc84781c94d109fb1f7fada0bfddd,
title = "Development and characterization of a novel human Waldenstr{\"o}m macroglobulinemia cell line: RPCI-WM1, Roswell Park Cancer Institute- Waldenstr{\"o}m Macroglobulinemia 1",
abstract = "Understanding the biology of Waldenstr{\"o}m macroglobulinemia is hindered by a lack of preclinical models. We report a novel cell line, RPCI-WM1, from a patient treated for WM. The cell line secretes human immunoglobulin M (h-IgM) with κ-light chain restriction identical to the primary tumor. The cell line has a modal chromosomal number of 46 and harbors chromosomal changes such as deletion of 6q21, monoallelic deletion of 9p21 (CDKN2A), 13q14 (RB1) and 18q21 (BCL-2), with a consistent amplification of 14q32 (immunoglobulin heavy chain; IgH) identical to its founding tumor sample. The clonal relationship is confirmed by identical CDR3 length and single nucleotide polymorphisms as well as a matching IgH sequence of the cell line and founding tumor. Both also harbor a heterozygous, non-synonymous mutation at amino acid 265 in the MYD88 gene (L265P). The cell line expresses most of the cell surface markers present on the parent cells. Overall, RPCI-WM1 represents a valuable model to study Waldenstr{\"o}m macroglobulinemia.",
keywords = "Cell line, Model, Preclinical, Waldenstr{\"o}m macroglobulinemia",
author = "Chitta, {Kasyapa S.} and Aneel Paulus and Sikander Ailawadhi and Foster, {Barbara A.} and Moser, {Michael T.} and Petr Starostik and Aisha Masood and Taimur Sher and Miller, {Kena C.} and Iancu, {Dan M.} and Jeffrey Conroy and Nowak, {Norma J.} and Sait, {Sheila N.} and Personett, {David A.} and Morton Coleman and Furman, {Richard R.} and Peter Martin and Ansell, {Stephen M.} and Kelvin Lee and Chanan-Khan, {Asher A.}",
note = "Funding Information: We are very grateful to the patient and her family for graciously donating her tumor tissue. This work is partly supported by the Waterfall Waldenstr{\"o} m Macroglobulinemia Research Fund (A.C.-K.), the Leukemia and Lymphoma Society (A.C.-K. is a Leukemia and Lymphoma Society Scholar in Clinical Research), and R01 CA121044-01 and R01 CA097243 (K.L. and N.J.N.). This research was also supported, in part, by a NCI Cancer Center Support Grant to the Roswell Park Cancer Institute (P30 CA016056) (N.J.N.). Additional support was provided in part by the Roswell Park Alliance Foundation, “ Rapid Tissue Acquisition Program” and the Roswell Park Cancer Institute Shared Resource, “ Mouse Tumor Model Resource (MTMR) ” (B.A.F.). This work was also supported by grant support from the international Walden-str{\"o}m macroglobulinemia foundation (IWMF) and the leukemia and lymphoma society (A.C.-K.). We are grateful to Dr. Kiersten Marie Miles for her contribution to this project.",
year = "2013",
month = feb,
doi = "10.3109/10428194.2012.713481",
language = "English (US)",
volume = "54",
pages = "387--396",
journal = "Leukemia and Lymphoma",
issn = "1042-8194",
publisher = "Informa Healthcare",
number = "2",
}